Hayden M S, Gilliland L K, Ledbetter J A
Department of Autoimmunity and Transplantation, Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington, WA 98121, USA.
Curr Opin Immunol. 1997 Apr;9(2):201-12. doi: 10.1016/s0952-7915(97)80136-7.
The development of recombinant techniques for the rapid cloning, expression, and characterization of cDNAs encoding antibody (Ab) subunits has revolutionized the field of antibody engineering. By fusion to heterologous protein domains, chain shuffling, and inclusion of self-assembly motifs, novel molecules such as bispecific Abs can now be generated which possess the subset of functional properties designed to fit the intended application. Rapid technological developments in phage display of peptides and proteins have led to a plethora of applications directed towards immunology and antibody engineering. Many of the problems associated with the therapeutic use of Abs are being addressed by the application of these new techniques.
用于快速克隆、表达和鉴定编码抗体（Ab）亚基的cDNA的重组技术的发展，彻底改变了抗体工程领域。通过与异源蛋白结构域融合、链改组以及包含自组装基序，现在可以产生诸如双特异性抗体之类的新型分子，这些分子具有为适应预期应用而设计的功能特性子集。肽和蛋白质噬菌体展示技术的快速发展已导致大量针对免疫学和抗体工程的应用。这些新技术的应用正在解决许多与抗体治疗用途相关的问题。
