电鳐（Discopyge ommata）主要穹窿蛋白编码cDNA的分析。

Analysis of a cDNA encoding the major vault protein from the electric ray Discopyge ommata.

作者信息

Herrmann C, Zimmermann H, Volknandt W

机构信息

Biozentrum, J.W. Goethe-Universität, Zoologisches Institut, Frankfurt am Main, Germany.

出版信息

Gene. 1997 Mar 25;188(1):85-90. doi: 10.1016/s0378-1119(96)00781-0.

DOI:10.1016/s0378-1119(96)00781-0

PMID:9099863

Abstract

The major vault protein is the predominant constituent of vaults ubiquitous large cytosolic ribonucleoprotein particles. A cDNA clone encoding the 100-kDa major vault protein (MVP100) was isolated from an electric lobe library of Discopyge ommata. The complete nucleotide sequence was determined. Northern blot analysis revealed a 2.8-kb transcript with a high expression in neural tissue. Southern blot analysis indicates that the electric ray MVP100 is a single copy-gene with at least two introns. The primary structure of major vault proteins characterized in slime mold, ray, rat and human is evolutionary highly conserved.

摘要

主要穹窿蛋白是穹窿（普遍存在的大型胞质核糖核蛋白颗粒）的主要成分。从电鳐（Discopyge ommata）的电叶文库中分离出一个编码100 kDa主要穹窿蛋白（MVP100）的cDNA克隆。测定了其完整的核苷酸序列。Northern印迹分析显示有一个2.8 kb的转录本，在神经组织中高表达。Southern印迹分析表明，电鳐的MVP100是一个单拷贝基因，至少有两个内含子。在黏菌、电鳐、大鼠和人类中鉴定的主要穹窿蛋白的一级结构在进化上高度保守。