el-Barrawy M, Lichtiger B, Fisher H, Daryl O
Department of Immunology and Blood Bank, M.D. Anderson Cancer Center, Texas University, Houston 77030.
Hum Immunol. 1991 Mar;30(3):222-5. doi: 10.1016/0198-8859(91)90038-b.
The serological determination of class II antigens is still a mandatory test prior to allotransplantation. It is known that these antigens are normally expressed on B lymphocytes and monocytes. The B lymphocytes that constitute 10% to 15% of total blood lymphocytes are the cells currently used for HLA-DR typing. To avoid HLA-DR typing difficulties, or even impossibilities that are frequently encountered among some patient groups, we studied the response of peripheral blood mononuclear cells--as an alternative source of cells for class II antigen typing--to in vitro mitogen and interleukin-2 activation and propagation. Although the patients included in this study were selected having previously known HLA-DR typing difficulties, all could be adequately typed by this method.
在同种异体移植前，II类抗原的血清学检测仍然是一项强制性检测。众所周知，这些抗原通常在B淋巴细胞和单核细胞上表达。占血液淋巴细胞总数10%至15%的B淋巴细胞是目前用于HLA-DR分型的细胞。为避免在某些患者群体中经常遇到的HLA-DR分型困难甚至无法分型的情况，我们研究了外周血单个核细胞（作为II类抗原分型的替代细胞来源）对体外有丝分裂原和白细胞介素-2激活及增殖的反应。尽管本研究纳入的患者是先前已知存在HLA-DR分型困难的，但所有患者都可以通过这种方法进行充分分型。
