Sorimachi K, Akimoto K, Niwa A, Yasumura Y
Department of Microbiology, Dokkyo University School of Medicine, Tochigi, Japan.
Cancer Detect Prev. 1997;21(2):111-7.
When rat fibroblasts (Ad12-3Y1-Z19) transformed with adenovirus type 12 were cultured with lignin derivative (acetyl or sulfonyl), the cells grew for 2 to 3 days at the same rate as the control cells cultured without lignin derivative, then rapidly died. This cytocidal effect was independent of the cell population density. The lag time was longer than the doubling time (-24 h) of Ad12-3Y1-Z19 cells. Other polyanions such as dextran sulfate and glycosaminoglycans did not show significant inhibitory effect on Ad12-3Y1-Z19 cell growth. In order to determine whether or not this cytocidal effect is general for every cell line, we examined 14 cell lines derived from tumor tissues and normal tissues, and 8 cell lines transformed with viruses, chemical carcinogens, or oncogenes. Of these cell lines, many responded to lignin derivatives with inhibition of cell growth, while in some cell lines no inhibitory effect of lignin derivatives was observed. The cytocidal effect was observed in only Ad12-3Y1-Z19 cells. This may be a new type of cytocidal phenomenon.
用12型腺病毒转化的大鼠成纤维细胞（Ad12 - 3Y1 - Z19）与木质素衍生物（乙酰基或磺酰基）一起培养时，细胞最初2至3天的生长速度与未添加木质素衍生物培养的对照细胞相同，之后迅速死亡。这种细胞杀伤作用与细胞群体密度无关。延迟时间长于Ad12 - 3Y1 - Z19细胞的倍增时间（-24小时）。其他聚阴离子如硫酸葡聚糖和糖胺聚糖对Ad12 - 3Y1 - Z19细胞生长未显示出显著抑制作用。为了确定这种细胞杀伤作用是否对每种细胞系都普遍存在，我们检测了14种源自肿瘤组织和正常组织的细胞系，以及8种用病毒、化学致癌物或癌基因转化的细胞系。在这些细胞系中，许多细胞系对木质素衍生物有细胞生长抑制反应，而在一些细胞系中未观察到木质素衍生物的抑制作用。仅在Ad12 - 3Y1 - Z19细胞中观察到细胞杀伤作用。这可能是一种新型的细胞杀伤现象。
