Suzuki J, Inoue Y, Suzuki S
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Free Radic Biol Med. 1995 Mar;18(3):431-6. doi: 10.1016/0891-5849(94)00152-a.
A simple means of measuring of 8-hydroxyguanine (8-OHGua) levels in urine was developed. Rat and human urine samples were purified by means of strong cation exchange chromatography (Amberlite CG-120), followed by cellulose partition chromatography (Whatman CF-11). Thereafter, 8-OHGua was determined by means of high performance liquid chromatography (HPLC) using an electro-chemical detector. The level of 8-OHGua in rat urine increased by a factor of 2 to 4 after an intraperitoneal administration of 2-nitropropane (25 mg/kg), paraquat (11.3 mg/kg), or hydroquinone (11 mg/kg). On the other hand, the urine of smokers and persons exposed to air polluted with car exhaust also contained 1.9 and 3.8 fold more 8-OHGua, respectively, than that of control nonsmokers. These results indicated that the amount of 8-OHGua in urine is useful marker for monitoring the level of in vivo oxidative stress.
开发了一种简单的测量尿液中8-羟基鸟嘌呤（8-OHGua）水平的方法。大鼠和人类尿液样本通过强阳离子交换色谱法（Amberlite CG-120）进行纯化，随后进行纤维素分配色谱法（Whatman CF-11）。此后，使用电化学检测器通过高效液相色谱法（HPLC）测定8-OHGua。腹腔注射2-硝基丙烷（25 mg/kg）、百草枯（11.3 mg/kg）或对苯二酚（11 mg/kg）后，大鼠尿液中8-OHGua的水平增加了2至4倍。另一方面，吸烟者和暴露于汽车尾气污染空气中的人的尿液中8-OHGua的含量分别比对照非吸烟者多1.9倍和3.8倍。这些结果表明，尿液中8-OHGua的含量是监测体内氧化应激水平的有用标志物。
