Fetsch P A, Cormier J, Hijazi Y M
Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1500, USA.
J Immunother. 1997 Jan;20(1):60-4. doi: 10.1097/00002371-199701000-00006.
MART-1 is a transmembrane protein that appears to be melanocyte specific. This study evaluated the use of a new monoclonal antibody, directed against MART-1, in fresh and paraffin-embedded specimens. Twenty-three paraffin-embedded tumors were evaluated for MART-1 immunoreactivity both before and after microwave-based antigen retrieval. After this comparison, 53 fine-needle aspirates from 43 patients with malignant melanoma were assessed for MART-1 immunoreactivity. Immunocytochemistry was performed on both cytospins and paraffin-embedded tissues that were pretreated with antigen retrieval. Seventeen (74%) of 23 tumors evaluated for immunoreactivity before and after antigen retrieval showed a significant increase in both staining intensity and the number of cells stained. When cytospins and antigen-retrieved cell blocks from 53 fine-needle aspirates were compared. 38 (72%) showed good correlation. In 13 (25%) of 53 tumors, MART-1 immunoreactivity was more intense in the cytospins, although the differences were marked in only two cases: Microwave-based antigen retrieval renders paraffin-embedded tissues nearly as sensitive as fresh material for use in the immunocytochemical detection of MART-1. This technique will allow the evaluation of MART-1 immunoreactivity in archival malignant melanomas.
MART-1是一种似乎具有黑素细胞特异性的跨膜蛋白。本研究评估了一种针对MART-1的新型单克隆抗体在新鲜标本和石蜡包埋标本中的应用。对23个石蜡包埋肿瘤在基于微波的抗原修复前后进行MART-1免疫反应性评估。经过这种比较后，对来自43例恶性黑色素瘤患者的53份细针穿刺抽吸物进行MART-1免疫反应性评估。对经过抗原修复预处理的细胞涂片和石蜡包埋组织进行免疫细胞化学检测。在对23个肿瘤进行抗原修复前后免疫反应性评估时，17个（74%）肿瘤的染色强度和染色细胞数量均显著增加。当比较53份细针穿刺抽吸物的细胞涂片和经过抗原修复的细胞块时，38个（72%）显示出良好的相关性。在53个肿瘤中的13个（25%）中，MART-1免疫反应性在细胞涂片中更强，尽管只有两例差异明显：基于微波的抗原修复使石蜡包埋组织在用于MART-1的免疫细胞化学检测时几乎与新鲜材料一样敏感。这项技术将允许对存档的恶性黑色素瘤进行MART-1免疫反应性评估。
