Rolandelli R H, Buckmire M A, Bernstein K A
Philadelphia Veterans Administration Medical Center, Pennsylvania, USA.
Dis Colon Rectum. 1997 Jan;40(1):67-70. doi: 10.1007/BF02055684.
Intracolonic infusions of short chain fatty acids promote healing of colonic anastomoses. Because the intravenous route may have wider clinical application, we studied the effect of intravenous n-butyrate on the mechanical strength of colonic anastomoses in the rat.
After placement of an indwelling intravenous catheter, the descending colon was transected and an anastomosis was performed. Rats were then randomized to receive total parenteral nutrition (TPN group; n = 15) or total parenteral nutrition plus 130 mM/l of n-butyrate (TPN+BUT group; n = 13). On the fifth postoperative day, bursting pressure and bowel wall tension of the anastomoses were measured in situ. Anastomotic tissues were analyzed for hydroxyproline.
The TPN+BUT group had a significantly higher bursting pressure (107.5 +/- 30.3 vs. 83 +/- 41.0 mmHg; P = 0.04) and bowel wall tension (20.7 +/- 7.6 vs. 14.1 +/- 9.9 Newton; P = 0.03). Tissue hydroxyproline was not different between the two groups (TPN, 45.8 +/- 9.2, and TPN+BUT, 47.9 +/- 2.9 microg/mg tissue nitrogen).
We conclude that intravenous butyrate improves mechanical strength of a colonic anastomosis without a detectable change in total collagen content.
结肠内输注短链脂肪酸可促进结肠吻合口愈合。由于静脉途径可能具有更广泛的临床应用，我们研究了静脉注射丁酸盐对大鼠结肠吻合口机械强度的影响。
留置静脉导管后，横断降结肠并进行吻合。然后将大鼠随机分为接受全胃肠外营养（TPN组；n = 15）或全胃肠外营养加130 mM/l丁酸盐（TPN + BUT组；n = 13）。术后第5天，原位测量吻合口的爆破压力和肠壁张力。分析吻合组织中的羟脯氨酸。
TPN + BUT组的爆破压力（107.5±30.3对83±41.0 mmHg；P = 0.04）和肠壁张力（20.7±7.6对14.1±9.9牛顿；P = 0.03）显著更高。两组之间的组织羟脯氨酸无差异（TPN组为45.8±9.2，TPN + BUT组为47.9±2.9微克/毫克组织氮）。
我们得出结论，静脉注射丁酸盐可提高结肠吻合口的机械强度，而总胶原含量无明显变化。
