Andreeva L E, Grigorenko A P, Gordeeva O F, Dvorianchikov G A
Genetika. 1996 Dec;32(12):1661-8.
The bacterial gene for beta-galactosidase under the control of LTR from either human cytomegalovirus (CMV-lacZ) or the Rous sarcoma virus (RSV-lacZ) was injected into fertilized eggs of Misgurnus fossilis L. loaches and F1 hybrid mice (CBA x C57Bl). Expression of the CMV-lacZ was observed in almost 100% of the loach embryos and larvae for two months following the first day of embryonic development. In some cases, expression points were located only on either the right or the left side of a fish. The spectrum of tissues expressing CMV-lacZ was decreased during embryonic development: CMV-lacZ was expressed only in fin and body muscles of 6- to 8-week-old loaches. In mice, the RSV-lacZ gene was expressed in ectoderm- and mesoderm-derived tissues of a 13-day-old embryo, and the CMV-lacZ gene was expressed in tissues derived from various blastophylli of a 14-day-old embryo. Distribution of transgene expression is discussed with regard to authors' and published data.
将受人类巨细胞病毒（CMV - lacZ）或劳斯肉瘤病毒（RSV - lacZ）的长末端重复序列（LTR）控制的β - 半乳糖苷酶细菌基因，注射到泥鳅（Misgurnus fossilis L.）和F1杂交小鼠（CBA×C57Bl）的受精卵中。在胚胎发育第一天后的两个月内，在几乎100%的泥鳅胚胎和幼体中观察到CMV - lacZ的表达。在某些情况下，表达点仅位于鱼的右侧或左侧。在胚胎发育过程中，表达CMV - lacZ的组织范围缩小：CMV - lacZ仅在6至8周龄泥鳅的鳍和身体肌肉中表达。在小鼠中，RSV - lacZ基因在13日龄胚胎的外胚层和中胚层来源的组织中表达，而CMV - lacZ基因在14日龄胚胎的各种胚叶来源的组织中表达。结合作者和已发表的数据讨论了转基因表达的分布情况。
