Wagner J G, Hallmark M R, Sakmar E, Ayres J W
Steroids. 1977 Jun;29(6):787-807. doi: 10.1016/0039-128x(77)90122-2.
A reproducible and sensitive radioimmunoassay for digoxin in either serum, plasma or urine is described. Using 0.5 ml of serum or plasma, the assay sensitivity is 0.05 ng of digoxin/ml. The antiserum and tracer solutions employed are available in a kit sold in the United States. All other reagents were prepared in the laboratory. The assay allows measurement of digoxin in plasma or serum for 96 hours after single 0.5 mg doses of digoxin; this is necessary in human bioavailability studies to accurately estimate the total area under the digoxin concentration, time curve from zero to infinite time. In contrast, with the kit assay, employing 0.2 ml of plasma or serum, it has been reported that the 12 hr serum digoxin levels, after single 0.5 mg doses, are, in most subjects, below the sensitivity limit (about 0.5 ng/ml) of the assay.
本文描述了一种用于检测血清、血浆或尿液中地高辛的可重复且灵敏的放射免疫分析方法。使用0.5毫升血清或血浆时，该分析方法的灵敏度为每毫升0.05纳克地高辛。所使用的抗血清和示踪剂溶液可在美国销售的试剂盒中获得。所有其他试剂均在实验室制备。该分析方法能够在单次给予0.5毫克地高辛剂量后96小时内测定血浆或血清中的地高辛；这在人体生物利用度研究中对于准确估计地高辛浓度-时间曲线从零到无限时间的总面积是必要的。相比之下，据报道，使用该试剂盒分析方法，采用0.2毫升血浆或血清，在单次给予0.5毫克剂量后，大多数受试者在12小时时的血清地高辛水平低于该分析方法的灵敏度极限（约0.5纳克/毫升）。
