Brinkmann A O
Steroids. 1977 Jun;29(6):861-73. doi: 10.1016/0039-128x(77)90128-3.
Synthesis and secretion of testosterone by testes of guinea pig fetuses were studied in organ culture. The amount of testosterone secreted into the culture medium was estimated by radio-immunoassay. It was demonstrated that testosterone was already secreted by testes explanted at 25 days. The amount of testosterone secreted during the first day in culture by testes from fetuses of different ages (25-30 days) increased with fetal age. The amount of testosterone extracted from fetal guinea pig testes at ages used for explantation (25-30 days) were much lower than the amount secreted during the first 24 hours in culture. During subsequent days in culture, an increase in the amount of testosterone secreted was observed only for testes explanted at 25 days. The amount secreted by testes from older fetuses (26-30 days) stayed constant or decreased. Aminogluthetimide phosphate (100 micron) decreased significantly testosterone secretion by testes explanted at 25 days. These findings indicate that the capacity for biosynthesis of testosterone is present in fetal guinea pig testes at 25 days and increases during the subsequent days.
在器官培养中研究了豚鼠胎儿睾丸睾酮的合成与分泌。通过放射免疫测定法估算分泌到培养基中的睾酮量。结果表明，25天时移出的睾丸已能分泌睾酮。不同年龄（25 - 30天）胎儿的睾丸在培养第一天分泌的睾酮量随胎龄增加。用于移出培养（25 - 30天）的豚鼠胎儿睾丸中提取的睾酮量远低于培养最初24小时分泌的量。在培养的后续几天里，仅观察到25天时移出的睾丸分泌的睾酮量增加。来自较大胎儿（26 - 30天）的睾丸分泌量保持恒定或减少。氨基谷氨酰胺磷酸盐（100微米）显著降低了25天时移出的睾丸的睾酮分泌。这些发现表明，25天时豚鼠胎儿睾丸就具备睾酮生物合成能力，且在随后几天有所增加。
