Picon R
J Endocrinol. 1976 Nov;71(2):231-8. doi: 10.1677/joe.0.0710231.
Testosterone secretion by foetal rat testes (13 1/2-21 1/2 days of gestation) explanted for 3 days in a synthetic medium was measured every 24 h by radioimmunoassay. During the first day of explantation, the foetal testis produced, respectively, 1013 +/- 132, 8734 +/- 1118, 9179 +/- 2185 and 3886 +/- 309 (S.E.M.) pg/testis when explanted at 14 1/2, 16 1/2, 18 1/2 and 21 1/2 days respectively. Testosterone production by 13 1/2-day-old testes was not detectable on the first day of culture, but appeared on subsequent days. Daily testosterone secretion increased on the 2nd and 3rd days of culture in 14 1/2-day-old testes and decreased in older stages. These results suggest that the functional differentiation of the testis is independent of stimulatory factors like gonadotrophins. Dibutyryl cyclic AMP was found to stimulate testosterone production significantly from 14 1/2 days of gestation onwards.
采用放射免疫分析法，每24小时测量一次在合成培养基中培养3天的胎鼠睾丸（妊娠13.5 - 21.5天）的睾酮分泌量。在培养的第一天，胎龄分别为14.5、16.5、18.5和21.5天的睾丸组织移植后，每睾丸分别产生1013±132、8734±1118、9179±2185和3886±309（标准误）皮克睾酮。13.5日龄睾丸组织在培养第一天未检测到睾酮分泌，但在随后几天出现分泌。在14.5日龄睾丸组织中，培养第2天和第3天的每日睾酮分泌量增加，而在较大胎龄阶段则减少。这些结果表明，睾丸的功能分化独立于促性腺激素等刺激因素。发现二丁酰环磷酸腺苷从妊娠14.5天起能显著刺激睾酮分泌。
