Zhao M
Department of Hygiene, Kyushu University, Fukuoka.
Fukuoka Igaku Zasshi. 1997 Mar;88(3):49-55.
In this study, 124 mg/kg of allyl chloride (one-fifth of LD50) was administered to mice by a single subcutaneous injection and its effects on the male reproductive system were evaluated at 4 days, 9 days, 14 days, 22 days and 39 days after treatment. Allyl chloride decreased the weight of the testes, reduced the numbers of spermatid and sperm, and increased the frequency of the morphologically abnormal sperm without any sign of general influence. From these results, allyl chloride was confirmed to be a definite testicular toxicant in mice. Spermatid reduction was already apparent at 4 days after treatment. Therefore, it was found that allyl chloride affected the testis immediately after the injection. Sperm with an abnormal tail (most of them were sperm with a folded tail) increased at the cauda epididymis before the epididymal sperm reduction became apparent. Consequently, it seemed likely that, with its toxic effects on the testis, allyl chloride also affected sperm passing through the epididymis and disturbed their maturation. Spermatid/sperm reduction caused by allyl chloride did not recover and seemed to get worse until 39 days after treatment. The testicular damage caused by allyl chloride was irreversible during this observation period.
在本研究中，以124毫克/千克的烯丙基氯（半数致死量的五分之一）通过单次皮下注射给予小鼠，并在处理后的第4天、第9天、第14天、第22天和第39天评估其对雄性生殖系统的影响。烯丙基氯降低了睾丸重量，减少了精子细胞和精子的数量，并增加了形态异常精子的频率，且无任何全身影响的迹象。从这些结果来看，烯丙基氯被证实是小鼠明确的睾丸毒物。处理后第4天精子细胞减少就已很明显。因此，发现烯丙基氯在注射后立即影响睾丸。在附睾精子减少明显之前，附睾尾部异常尾巴的精子（大多数是尾巴折叠的精子）数量增加。因此，烯丙基氯对睾丸的毒性作用似乎也影响了通过附睾的精子并干扰了它们的成熟。烯丙基氯导致的精子细胞/精子减少直到处理后39天都没有恢复，而且似乎还在恶化。在该观察期内，烯丙基氯造成的睾丸损伤是不可逆的。
