Lee M Y, Naumann T, Kirsch M, Frotscher M, Hofmann H D
Institute of Anatomy, University of Freiburg, Germany.
Eur J Neurosci. 1997 Mar;9(3):622-6. doi: 10.1111/j.1460-9568.1997.tb01639.x.
Using non-radioactive in situ hybridization we investigated the effect of fimbria-fornix transection on the expression of ciliary neurotrophic factor receptor alpha (CNTFR alpha) mRNA in axotomized septohippocampal neurons of the rat septal complex. Whereas CNTFR alpha expression was undetectable in the medial septal nucleus/diagonal band complex (MSDB) of control animals, specific up-regulation was observed in MSDB neurons after fimbria-fornix transection. CNTFR alpha expression was maximal 7-10 days after the lesion and had returned to control levels after 3 weeks. Following unilateral fimbria-fornix transection, CNTFR alpha up-regulation was restricted to the MSDB ipsilateral to the lesion. When cholinergic septal neurons were selectively eliminated by immunolesioning with 192 IgG-saporin prior to fimbria-fornix transection, the lesion-induced expression of CNTFR alpha was still observed in many medial septal nucleus neurons. These results demonstrate that after fimbria-fornix transection CNTFR alpha expression is transiently induced in axotomized, non-cholinergic neurons of the medial septal nucleus, suggesting a postlesion function of locally supplied CNTF.
我们采用非放射性原位杂交技术，研究了穹窿海马伞横断对大鼠隔复合体中轴突切断的隔海马神经元睫状神经营养因子受体α（CNTFRα）mRNA表达的影响。在对照动物的内侧隔核/斜角带复合体（MSDB）中未检测到CNTFRα表达，而在穹窿海马伞横断后的MSDB神经元中观察到特异性上调。CNTFRα表达在损伤后7 - 10天达到最大值，并在3周后恢复到对照水平。单侧穹窿海马伞横断后，CNTFRα上调仅限于损伤同侧的MSDB。在穹窿海马伞横断前用192 IgG-皂草素进行免疫损伤选择性消除胆碱能隔神经元后，在许多内侧隔核神经元中仍观察到损伤诱导的CNTFRα表达。这些结果表明，穹窿海马伞横断后，内侧隔核中轴突切断的非胆碱能神经元中CNTFRα表达被短暂诱导，提示局部供应的睫状神经营养因子（CNTF）在损伤后具有功能。
