A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody.
作者信息
Kobatake E, Sasakura H, Haruyama T, Laukkanen M L, Keinänen K, Aizawa M
机构信息
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
Anal Chem. 1997 Apr 1;69(7):1295-8. doi: 10.1021/ac961162+.
Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10(-7) to 10(-9) M.
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