Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea.
School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
Sensors (Basel). 2017 Jun 23;17(7):1480. doi: 10.3390/s17071480.
In this study, we prepared adenosine triphosphate (ATP) encapsulated liposomes, and assessed their applicability for the surface enhanced Raman scattering (SERS)-based assays with gold-silver alloy (Au@Ag)-assembled silica nanoparticles (NPs; SiO₂@Au@Ag). The liposomes were prepared by the thin film hydration method from a mixture of l-α-phosphatidylcholine, cholesterol, and PE-PEG2000 in chloroform; evaporating the solvent, followed by hydration of the resulting thin film with ATP in phosphate-buffered saline (PBS). Upon lysis of the liposome, the SERS intensity of the SiO₂@Au@Ag NPs increased with the logarithm of number of ATP-encapsulated liposomes after lysis in the range of 8 × 10⁶ to 8 × 10. The detection limit of liposome was calculated to be 1.3 × 10 mol. The successful application of ATP-encapsulated liposomes to SiO₂@Au@Ag NPs based SERS analysis has opened a new avenue for Raman label chemical (RCL)-encapsulated liposome-enhanced SERS-based immunoassays.
在这项研究中，我们制备了三磷酸腺苷（ATP）包封的脂质体，并评估了其在基于金-银合金（Au@Ag）组装的硅纳米颗粒（NPs；SiO₂@Au@Ag）的表面增强拉曼散射（SERS）测定中的适用性。脂质体通过薄膜水化法从氯仿中的 l-α-磷脂酰胆碱、胆固醇和 PE-PEG2000 的混合物中制备；蒸发溶剂，然后用 PBS 中的 ATP 水化所得薄膜。在脂质体裂解后，SiO₂@Au@Ag NPs 的 SERS 强度随着裂解后包裹的 ATP 脂质体数量的对数增加，范围为 8×10⁶ 至 8×10。脂质体的检测限计算为 1.3×10 mol。ATP 包封脂质体在基于 SiO₂@Au@Ag NPs 的 SERS 分析中的成功应用为拉曼标记化学（RCL）包封脂质体增强的基于 SERS 的免疫分析开辟了新途径。
