对在贝尔格莱德地区采集的不同花粉季节的梯牧草（早熟禾）花粉的致敏潜力进行比较。

Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area.

作者信息

Gavrović M D, Trtić T, Vujcić Z, Petrović S, Jankov R M

机构信息

Faculty of Chemistry, Belgrade, Yugoslavia.

出版信息

Allergy. 1997 Feb;52(2):210-4. doi: 10.1111/j.1398-9995.1997.tb00977.x.

DOI:10.1111/j.1398-9995.1997.tb00977.x

PMID:9105527

Abstract

We investigated extracts of timothy grass pollen from four seasons (1989, 1990, 1991, and 1994) by protein content, SDS-PAGE, immunoblot, RAST, RAST inhibition, and crossed immunoelectrophoresis. Extract of the pollen from 1991 showed the lowest yield in quantitative assays. SDS-PAGE, crossed immunoelectrophoresis, RAST, and RAST inhibition expressed approximately comparable patterns for all extracts except that from 1991. Obviously, the quality of grass pollens, as shown for some ragweed (Ambrosia elatior) pollens depend on year of collection. Our findings are partially in agreement with some earlier examinations of the quality of timothy pollen from different pollen seasons.

摘要

我们通过蛋白质含量、SDS - 聚丙烯酰胺凝胶电泳、免疫印迹、放射变应原吸附试验、放射变应原吸附试验抑制以及交叉免疫电泳，对四个季节（1989年、1990年、1991年和1994年）的梯牧草花粉提取物进行了研究。1991年花粉提取物在定量分析中产量最低。除1991年的提取物外，SDS - 聚丙烯酰胺凝胶电泳、交叉免疫电泳、放射变应原吸附试验以及放射变应原吸附试验抑制对所有提取物显示出大致可比的模式。显然，正如一些豚草（Ambrosia elatior）花粉所显示的那样，禾本科花粉的质量取决于采集年份。我们的研究结果部分与之前对不同花粉季节梯牧草花粉质量的一些检测结果一致。