Pedersen L H, Skouboe P, Boysen M, Soule J, Rossen L
Biotechnological Institute, Lyngby, Denmark.
Int J Food Microbiol. 1997 Apr 1;35(2):169-77. doi: 10.1016/s0168-1605(96)01216-0.
Rapid identification of filamentous fungi is becoming increasingly important in food mycology both for monitoring the production process and for the identification of food spoilers. This paper describes the development and trial of two specific PCR primer sets. A 336 bp fragment from species belonging to Penicillium subgenus Penicillium was amplified by the primers ITS 212d and ITS 549. The other primer set, ITS 183 and ITS 401 specifically identified two species, Penicillium roqueforti and P. carneum, both known as spoilers in the bread industry, by amplification of a 300 bp fragment. The future perspectives of PCR based identification of filamentous fungi in food are discussed.
在食品真菌学中，快速鉴定丝状真菌对于监测生产过程和识别食品腐败菌变得越来越重要。本文描述了两种特异性PCR引物组的开发和试验。引物ITS 212d和ITS 549扩增出了来自青霉亚属青霉的336 bp片段。另一组引物ITS 183和ITS 401通过扩增300 bp片段，特异性鉴定了两种在面包行业中均为腐败菌的真菌——罗克福特青霉和肉色青霉。本文还讨论了基于PCR技术鉴定食品中丝状真菌的未来前景。
