Alvarez J G, Slomovic B, Ludmir J
Department of Obstetrics and Gynecology, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215, USA.
J Chromatogr B Biomed Sci Appl. 1997 Mar 7;690(1-2):338-42. doi: 10.1016/s0378-4347(96)00415-x.
A novel method for the determination of dipalmitoyl phosphatidylcholine (DPPC) in amniotic fluid by high-performance liquid chromatography (HPLC) is described. Aliquots of 50 microliters of amniotic fluid were hydrolyzed with phospholipase C from Bacillus cereus and the resulting dipalmitoylglycerol analyzed by HPLC. Run-to-run and day-to-day precision for DPPC analysis were 4.2 and 6.1%, respectively, and analysis time was 10 min. Recoveries for DPPC ranged between 92 and 98%. In summarizing, this method provides a high precision and fast turnaround time means for the analysis of DPPC in amniotic fluid.
描述了一种通过高效液相色谱（HPLC）测定羊水二棕榈酰磷脂酰胆碱（DPPC）的新方法。取50微升羊水等分试样，用蜡样芽孢杆菌的磷脂酶C进行水解，所得二棕榈酰甘油通过HPLC分析。DPPC分析的批内和批间精密度分别为4.2%和6.1%，分析时间为10分钟。DPPC的回收率在92%至98%之间。总之，该方法为羊水中DPPC的分析提供了一种高精度且周转时间短的手段。
