对皮下注射一氧化氮结合铁络合物的小鼠肝脏进行体内电子自旋共振计算机断层成像。

In vivo ESR-CT imaging of the liver in mice receiving subcutaneous injection of nitric oxide-bound iron complex.

作者信息

Yokoyama H, Fujii S, Yoshimura T, Ohya-Nishiguchi H, Kamada H

机构信息

Institute for Life Support Technology, Yamagata Technopolis Foundation, Japan.

出版信息

Magn Reson Imaging. 1997;15(2):249-53. doi: 10.1016/s0730-725x(96)00350-5.

DOI:10.1016/s0730-725x(96)00350-5

PMID:9106153

Abstract

ESR measurements and ESR-CT imaging of subcutaneously (SC) injected nitric oxide (NO)-bound iron complexes were conducted on the upper abdomen of live mice. The use of NO-bound iron complex with N-(dithiocarboxy)sarcosine resulted in a clear ESR-CT image showing high intensity areas in the ventral regions, while other NO-bound iron complexes with N-methyl-D-glucamine dithiocarbamate or N,N-diethyl-dithiocarbamate were inappropriate because of low S/N ratios. To investigate the distributions of SC injected NO-bound iron complexes in the abdomens of mice, we measured ESR signals in resected abdominal organs. The signal amplitude was higher in the resected liver than in the resected gastrointestinal organs or the blood samples. The findings suggest that the high intensity areas in the ESR-CT images thus obtained correspond to the liver.

摘要

对皮下（SC）注射一氧化氮（NO）结合铁配合物的活体小鼠上腹部进行了电子自旋共振（ESR）测量和ESR计算机断层扫描（ESR-CT）成像。使用与N-（二硫代羧基）肌氨酸结合的NO铁配合物可得到清晰的ESR-CT图像，显示腹部区域有高强度区域，而其他与N-甲基-D-葡萄糖胺二硫代氨基甲酸盐或N，N-二乙基二硫代氨基甲酸盐结合的NO铁配合物因信噪比低而不合适。为了研究皮下注射的NO结合铁配合物在小鼠腹部的分布，我们测量了切除的腹部器官中的ESR信号。切除肝脏中的信号幅度高于切除的胃肠道器官或血液样本中的信号幅度。这些发现表明，由此获得的ESR-CT图像中的高强度区域对应于肝脏。