Habermalz F
Microsc Acta. 1979 Jul;82(1):11-24.
When comparing spectral and colorimetric data of colors of our natural environment with the data of microscopical stains, we observe considerable differences in spectral transmittance respectively reflectance. Furthermore, the spectral locus of these colors are situated at very different positions in the color space or in a chromaticity diagram. By this reason, we must anticipate that usual color reversal materials being optimized in the very first line for the reproduction of colors like foliage green, sky blue or earth, cannot render the hues of histological stains in a satisfying manner. To prove this assertion, the reproducible ranges of hues in the color space were calculated by means of the spectral densities of the dyes developed in the three layers of various color reversal films, and these results were then compared with the spectral data of some stains often applied for histology (H-E, Goldner, Azan etc.). By this method, we find that no color film examined can reproduce in a sufficient manner all these stains at the same time. The results can allow for color micrography the selection of a definite film for best possible color matching of a least one interesting range of color.
当我们将自然环境颜色的光谱数据和比色数据与显微镜染色的数据进行比较时，会观察到光谱透射率和反射率存在相当大的差异。此外，这些颜色的光谱轨迹在颜色空间或色度图中位于非常不同的位置。因此，我们必须预料到，通常首先针对诸如叶绿色、天蓝色或泥土色等颜色的再现进行优化的彩色反转材料，无法令人满意地呈现组织学染色的色调。为了证明这一断言，通过各种彩色反转胶片三层中所显影染料的光谱密度，计算出颜色空间中可再现的色调范围，然后将这些结果与一些组织学常用染色剂（苏木精-伊红染色、戈德纳染色、阿赞染色等）的光谱数据进行比较。通过这种方法，我们发现所检查的彩色胶片都无法同时充分再现所有这些染色剂。这些结果可以为彩色显微摄影提供参考，以便选择一种确定的胶片，以实现至少一个感兴趣颜色范围的最佳颜色匹配。
