Kaplan O, Ruíz-Cabello J, Cohen J S
Department of Surgery A, Tel-Aviv Medical Center, Israel.
FEBS Lett. 1997 Apr 7;406(1-2):175-8. doi: 10.1016/s0014-5793(97)00265-2.
Tumor necrosis factor-alpha inhibited growth of cultured MCF-7 human breast cancer cells in a dose dependent manner. Tumor necrosis factor-alpha also markedly increased glucose consumption, and its cytotoxicity was modified by glucose concentrations in the growth medium; higher glucose levels were associated with increased cell survival. However, when the cells were perfused in physiological conditions, very high levels of tumor necrosis factor-alpha (200 ng/ml) in the perfusion solution had no inhibitory effects. Moreover, tumor necrosis factor-alpha had no effects on 31P nuclear magnetic resonance spectra of the perfused cells. In the traditional growth inhibition assays, cells are incubated for several days with a drug, a situation where their metabolism is altered due to the depletion of nutrients, the accumulation of toxic waste materials and pH changes. Perfusion experiments are more relevant to in vivo conditions, and may be used for studying metabolic processes and the mechanisms of action of therapeutic agents.
肿瘤坏死因子-α以剂量依赖方式抑制培养的MCF-7人乳腺癌细胞的生长。肿瘤坏死因子-α还显著增加葡萄糖消耗，其细胞毒性受生长培养基中葡萄糖浓度的影响；较高的葡萄糖水平与细胞存活率增加相关。然而，当细胞在生理条件下进行灌注时，灌注溶液中非常高浓度的肿瘤坏死因子-α（200 ng/ml）没有抑制作用。此外，肿瘤坏死因子-α对灌注细胞的31P核磁共振谱没有影响。在传统的生长抑制试验中，细胞与药物一起孵育数天，在这种情况下，由于营养物质的消耗、有毒废物的积累和pH变化，它们的代谢会发生改变。灌注实验与体内条件更相关，可用于研究代谢过程和治疗药物的作用机制。
