McGinnis M Y, Kahn D F
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, CUNY, New York, New York 10029, USA.
Horm Behav. 1997 Feb;31(1):15-23. doi: 10.1006/hbeh.1997.1367.
Castrated male rats received testosterone (T) via silastic capsules and bilateral implants of either anisomycin (ANI) in a cocoa butter/beeswax mixture or cocoa butter/beeswax alone as a control. Cannulae were placed directly into the medial preoptic area (MPOA) to determine if protein synthesis in this brain region is required for the expression of male sexual behavior. In the first experiment, using a restoration paradigm, it was found that a 25% mixture of ANI prevented restoration of male sexual behavior, whereas 4% ANI was only partially effective. In a second experiment, the ability of 25% ANI to suppress male sexual behavior in a maintenance paradigm was tested and found to be completely ineffective. In a third experiment, a 50% ANI dose was used and this dose significantly suppressed male sexual behavior by the fourth week. Sexual behavior returned following ANI discontinuation. Males receiving 50% ANI were also tested for sexual motivation (measured in a place preference test), as well as for scent marking and vocalizations. ANI implanted into the MPOA did not affect sexual motivation, as males in both ANI and control groups spent more time with a sexually receptive female than with a nonreceptive female. ANI in the MPOA did not affect scent marking, but significantly decreased ultrasonic vocalizations. These results suggest that, in rats, protein synthesis in the MPOA is required for the expression of male sexual behavior and vocalizations, but not for sexual preference or scent marking.
去势雄性大鼠通过硅橡胶胶囊接受睾酮（T），并双侧植入茴香霉素（ANI）于可可脂/蜂蜡混合物中或仅植入可可脂/蜂蜡作为对照。将套管直接插入内侧视前区（MPOA），以确定该脑区的蛋白质合成是否是雄性性行为表达所必需的。在第一个实验中，采用恢复范式，发现25%的ANI混合物可阻止雄性性行为的恢复，而4%的ANI仅部分有效。在第二个实验中，测试了25%的ANI在维持范式中抑制雄性性行为的能力，发现其完全无效。在第三个实验中，使用了50%的ANI剂量，到第四周时，该剂量显著抑制了雄性性行为。停止使用ANI后，性行为恢复。接受50%ANI的雄性大鼠还接受了性动机测试（通过位置偏好测试测量）以及气味标记和发声测试。植入MPOA的ANI不影响性动机，因为ANI组和对照组的雄性大鼠与性接受雌性大鼠相处的时间都比与非接受雌性大鼠相处的时间长。MPOA中的ANI不影响气味标记，但显著减少了超声波发声。这些结果表明，在大鼠中，MPOA中的蛋白质合成是雄性性行为和发声表达所必需的，但不是性偏好或气味标记所必需的。
