Sipos M L, Nyby J G
Department of Biological Sciences, Lehigh University, Bethlehem, PA 18015, USA.
Brain Res. 1996 Aug 5;729(1):29-44.
Cannulae containing testosterone proprionate (T) were bilaterally implanted into the medial preoptic area (MPO), the ventral tegmental area (VTA), or both areas concurrently (MPO/VTA) of castrated male house mice. A fourth group of castrates in which the intracranial implants missed their targets (MIMP) served as controls. In addition, other castrates were implanted subcutaneously with empty silastic capsules (BSIL) or silastic capsules containing T (TSIL). All subjects were examined for the following male-typical behaviors: mounting, attraction to female urine, ultrasonic mating vocalizations and urinary scent marking. In addition, the males were tested for activity levels to insure that they were not motorically impaired. In general, TSIL implants restored all male-typical behaviors to normal levels, whereas BSIL and MIMP implants were generally ineffective. Similar to previous findings, MPO implants alone completely restored ultrasonic vocalizations, partially restored urine marking, and had little or no effect upon mounting or urine preference. In contrast, VTA implants alone were ineffective at restoring any of these male-typical behaviors. However, the combined MPO/VTA implants were the most effective in restoring male-typical behaviors. In fact, a synergism between concurrent hormone action in the MPO and VTA was seen for mounting and urine preference. We interpret these data to indicate that androgen may act simultaneously in the MPO and VTA for more complete expression of some male-typical reproductive behaviors.
将含有丙酸睾酮（T）的套管双侧植入去势雄性家鼠的内侧视前区（MPO）、腹侧被盖区（VTA）或同时植入这两个区域（MPO/VTA）。第四组去势鼠的颅内植入物未命中目标（MIMP）作为对照。此外，其他去势鼠皮下植入空的硅橡胶胶囊（BSIL）或含有T的硅橡胶胶囊（TSIL）。对所有实验对象进行以下雄性典型行为的检测：爬跨、对雌性尿液的吸引力、超声交配叫声和尿液气味标记。此外，对雄性进行活动水平测试，以确保它们没有运动障碍。总体而言，TSIL植入物将所有雄性典型行为恢复到正常水平，而BSIL和MIMP植入物通常无效。与之前的研究结果相似，单独的MPO植入物完全恢复了超声叫声，部分恢复了尿液标记，对爬跨或尿液偏好几乎没有影响。相比之下，单独的VTA植入物在恢复这些雄性典型行为方面无效。然而，联合的MPO/VTA植入物在恢复雄性典型行为方面最有效。事实上，在MPO和VTA中同时发生的激素作用之间在爬跨和尿液偏好方面存在协同作用。我们对这些数据的解释是，雄激素可能在MPO和VTA中同时起作用，以使某些雄性典型生殖行为更完整地表达。
