Bendixen P H
Am J Vet Res. 1977 Aug;38(8):1161-2.
Four cows with persistent diarrhea and complement-fixing antibodies to johnin were examined, using the leukocyte-migration agarose test (LMAT) with johnin as the specific antigen. The diagnosis of paratuberculosis (Johne's disease) was microscopically verified at necropsy. As control animals, 3 healthy cows and 3 cows with disease other than paratuberculosis were examined, using LMAT. The migration indices in the group of cows with paratuberculosis were significantly lower (less than 0.001) than those in the group of control cows. Furthermore, the migration zones of the unstimulated peripheral blood leukocytes in the former group were significantly smaller (less than 0.001) than those of the control group.
对4头患有持续性腹泻且对副结核菌素具有补体结合抗体的奶牛进行了检测，以副结核菌素作为特异性抗原，采用白细胞迁移琼脂糖试验（LMAT）。在尸检时通过显微镜检查证实了副结核病（Johne氏病）的诊断。作为对照动物，使用LMAT对3头健康奶牛和3头患有非副结核性疾病的奶牛进行了检测。患有副结核病的奶牛组的迁移指数显著低于（小于0.001）对照奶牛组。此外，前一组未受刺激的外周血白细胞的迁移区明显小于（小于0.001）对照组。
