Mikhova V T, V"rbanov G P
Klin Lab Diagn. 1993 May-Jun(3):17-9.
Lipoprotein X is a reliable indicator of cholestasis. The present paper describes a simple method for direct measurement of lipoprotein X. Low-density lipoproteins, very low density lipoproteins, chylomicrons and abnormal lipoprotein X are precipitated from the serum by treatment with a solution of polyethylene glycol (PEG) MW 6000. Then phosphotungstic acid and PEG 6000 solution are added to the precipitate. The lipoprotein X is in the supernatant, where its level can be estimated from the content of phospholipids and cholesterol. This method permits the determination of lipoprotein X in several samples simultaneously, requires no sophisticated equipment, is sensitive, reproducible, accurate, and cheap.
脂蛋白X是胆汁淤积的可靠指标。本文描述了一种直接测定脂蛋白X的简单方法。通过用分子量为6000的聚乙二醇（PEG）溶液处理，可从血清中沉淀出低密度脂蛋白、极低密度脂蛋白、乳糜微粒和异常脂蛋白X。然后向沉淀物中加入磷钨酸和PEG 6000溶液。脂蛋白X存在于上清液中，可根据磷脂和胆固醇的含量估算其水平。该方法可同时测定多个样品中的脂蛋白X，无需复杂设备，具有灵敏、可重复、准确且成本低廉的特点。
