Talafant E, Továrek J
J Clin Chem Clin Biochem. 1981 Mar;19(3):155-7. doi: 10.1515/cclm.1981.19.3.155.
Non-esterified cholesterol was determined enzymatically with the cholesterol oxidase-peroxidase reaction using phenol and 4-aminophenazone as chromogens. This assay as done in the supernatant containing the lipoprotein-X. Other serum lipoproteins were removed by precipitation with heparin and zinc acetate followed by centrifugation. No standardization was required, the lipoprotein-X concentration being calculated with the use of the constant.
