Haneji N, Nakamura T, Takio K, Yanagi K, Higashiyama H, Saito I, Noji S, Sugino H, Hayashi Y
Department of Pathology, Tokushima University School of Dentistry, 3 Kuramotocho, Tokushima 770, Japan.
Science. 1997 Apr 25;276(5312):604-7. doi: 10.1126/science.276.5312.604.
It is unclear whether organ-specific autoantigens are critical for the development of primary Sjögren's syndrome (SS). A 120-kilodalton organ-specific autoantigen was purified from salivary gland tissues of an NFS/sld mouse model of human SS. The amino-terminal residues were identical to those of the human cytoskeletal protein alpha-fodrin. The purified antigen induced proliferative T cell responses and production of interleukin-2 and interferon-gamma in vitro. Neonatal immunization with the 120-kilodalton antigen prevented the disease in mice. Sera from patients with SS reacted positively with purified antigen and recombinant human alpha-fodrin protein, whereas those from patients with systemic lupus erythematosus and rheumatoid arthritis did not. Thus, the immune response to 120-kilodalton alpha-fodrin could be important in the initial development of primary SS.
尚不清楚器官特异性自身抗原对原发性干燥综合征（SS）的发病是否至关重要。从人类SS的NFS/sld小鼠模型的唾液腺组织中纯化出一种120千道尔顿的器官特异性自身抗原。其氨基末端残基与人细胞骨架蛋白α-血影蛋白的残基相同。纯化的抗原因体外诱导增殖性T细胞反应以及白细胞介素-2和干扰素-γ的产生。用120千道尔顿抗原对新生小鼠进行免疫可预防其发病。SS患者的血清与纯化抗原和重组人α-血影蛋白呈阳性反应，而系统性红斑狼疮和类风湿关节炎患者的血清则无此反应。因此，针对120千道尔顿α-血影蛋白的免疫反应可能在原发性SS的初始发病过程中起重要作用。
