Greene C N, Jinks-Robertson S
Graduate Program in Genetics and Molecular Biology, Emory University, Atlanta, Georgia 30322, USA.
Mol Cell Biol. 1997 May;17(5):2844-50. doi: 10.1128/MCB.17.5.2844.
A change in the number of base pairs within a coding sequence can result in a frameshift mutation, which almost invariably eliminates the function of the encoded protein. A frameshift reversion assay with Saccharomyces cerevisiae that can be used to examine the types of insertions and deletions that are generated during DNA replication, as well as the editing functions that remove such replication errors, has been developed. Reversion spectra have been obtained in a wild-type strain and in strains defective for defined components of the postreplicative mismatch repair system (msh2, msh3, msh6, msh3 msh6, pms1, and mih1 mutants). Comparison of the spectra reveals that yeast mismatch repair proteins preferentially remove frameshift intermediates that arise in homopolymer tracts and indicates that some of the proteins have distinct substrate or context specificities.
编码序列中碱基对数量的变化可导致移码突变，这种突变几乎总是会消除所编码蛋白质的功能。已经开发出一种酿酒酵母移码回复试验，可用于检测DNA复制过程中产生的插入和缺失类型，以及消除此类复制错误的编辑功能。在野生型菌株以及复制后错配修复系统特定组分缺陷的菌株（msh2、msh3、msh6、msh3 msh6、pms1和mih1突变体）中获得了回复谱。对这些谱的比较表明，酵母错配修复蛋白优先去除同聚物区域中出现的移码中间体，并表明其中一些蛋白质具有不同的底物或上下文特异性。
