Nakajima H, Kimura F, Nakagawa T, Ikemoto T, Furutama D, Shinoda K, Kato S, Shimizu A, Ohsawa N
First Department of Internal Medicine, Osaka Medical College, Takatsuki City, Japan.
Neurosci Lett. 1997 Jan 31;222(2):83-6. doi: 10.1016/s0304-3940(97)13348-1.
Expansion of the polyglutamine tracts in the androgen receptor (AR) has been recognized as a cause of X-linked spinal and bulbar muscular atrophy (SBMA). In the present study, NG108-15 cells were stably transfected with expression vectors coding for either the wild type (WT) AR gene (CAG repeat number = 22) or a mutated (MT) AR gene (CAG repeat number = 52). Cells proliferation and cell cycle parameters were evaluated for NG108-15-WT and NG108-15-MT cells in the presence or absence of androgen. NG108-15-WT cells demonstrated an androgen-dependent increase in cell number, while NG108-15-MT cells did not. Our results demonstrate that expansion of polyglutamine tracts in the AR may affect the proliferation and differentiation of nerve cells.
雄激素受体（AR）中聚谷氨酰胺序列的扩增已被确认为X连锁脊髓和延髓性肌萎缩症（SBMA）的病因。在本研究中，用编码野生型（WT）AR基因（CAG重复次数 = 22）或突变型（MT）AR基因（CAG重复次数 = 52）的表达载体稳定转染NG108-15细胞。在有或没有雄激素存在的情况下，评估NG108-15-WT和NG108-15-MT细胞的细胞增殖和细胞周期参数。NG108-15-WT细胞显示出雄激素依赖性的细胞数量增加，而NG108-15-MT细胞则没有。我们的结果表明，AR中聚谷氨酰胺序列的扩增可能会影响神经细胞的增殖和分化。
