Nakagawa H, Okada H, Fujisawa M, Matsumoto O, Kamidono S
Department of Urology, Kobe University School of Medicine, Japan.
Andrologia. 1997 Mar-Apr;29(2):103-8. doi: 10.1111/j.1439-0272.1997.tb00471.x.
Acrosin activity of spermatozoa from infertile patients and fertile volunteers was measured. Acrosin activity of spermatozoa from asthenozoospermic patients and patients with unexplained infertility was lower than that from fertile volunteers. We utilize the zona-free hamster egg-sperm penetration test to select candidates for conventional in vitro fertilization among unexplained infertile patients. The zona-free hamster egg sperm penetration test, however, requires several hours and special equipment which are not used in the clinical setting. It is preferable that other sperm function tests or a combination of them can replace this test. Thus three distinct tests of sperm function, namely, acrosin activity, Penetrak test, hypo-osmotic swelling test, were compared with the hamster test, using spermatozoa from patients with unexplained infertility. A combination of the Penetrak test and measurement of acrosin activity could predict the results of the zona-free hamster egg sperm penetration test with 88.2% accuracy. Thus, the hamster test should be done when either Penetrak test or measurement of acrosin activity showed abnormal values.
对不育患者和有生育能力志愿者的精子顶体酶活性进行了测定。弱精子症患者和不明原因不孕症患者的精子顶体酶活性低于有生育能力的志愿者。我们利用无透明带仓鼠卵-精子穿透试验在不明原因不孕症患者中选择常规体外受精的候选者。然而，无透明带仓鼠卵-精子穿透试验需要数小时且需要特殊设备，而这些在临床环境中并不常用。最好是其他精子功能测试或它们的组合能够替代该测试。因此，使用不明原因不孕症患者的精子，将三种不同的精子功能测试，即顶体酶活性、Penetrak试验、低渗肿胀试验，与仓鼠试验进行了比较。Penetrak试验和顶体酶活性测定的组合能够以88.2%的准确率预测无透明带仓鼠卵-精子穿透试验的结果。因此，当Penetrak试验或顶体酶活性测定显示异常值时，应进行仓鼠试验。
