Morikawa S, Kurauchi O, Tanaka M, Yoneda M, Uchida K, Itakura A, Furugori K, Mizutani S, Tomoda Y
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Nagoya, Japan.
Biochem Mol Biol Int. 1997 Apr;41(4):767-75. doi: 10.1080/15216549700201801.
Lipid peroxides and their related free radicals have been implicated in the pathogenesis of placental dysfunction in preeclampsia. Recent studies suggest that the placenta is a source of the increased lipid peroxides in the maternal circulation of women with preeclampsia. We examined intracellular localization of 4-hydroxy-2-nonenal (HNE: a major aldehydic product of lipid peroxidation)-modified proteins in human placentas by immunohistochemistry, and immunoblotting. The trophoblast layer of the chorionic villi showed intense immunoreactivity for HNE-modified proteins in 4 of 12 preeclamptic placentas, whereas no staining was observed in 12 normal placentas. Immunoblotting revealed that three immunoreactive proteins with apparent molecular mass of 110 kDa, 75 kDa, and 70 kDa were localized in the mitochondrial fraction. The present results indicate that the damage to mitochondrial proteins by lipid peroxidation by products and subsequent dysfunction of trophoblasts contribute to the pathophysiology of preeclampsia.
脂质过氧化物及其相关自由基与子痫前期胎盘功能障碍的发病机制有关。最近的研究表明，胎盘是子痫前期妇女母体循环中脂质过氧化物增加的来源。我们通过免疫组织化学和免疫印迹法检测了人胎盘中4-羟基-2-壬烯醛（HNE：脂质过氧化的主要醛类产物）修饰蛋白的细胞内定位。在12例子痫前期胎盘中，有4例绒毛膜绒毛的滋养层对HNE修饰蛋白显示出强烈的免疫反应性，而12例正常胎盘中未观察到染色。免疫印迹显示，三种表观分子量为110 kDa、75 kDa和70 kDa的免疫反应性蛋白定位于线粒体部分。目前的结果表明，脂质过氧化产物对线粒体蛋白的损伤以及随后滋养层细胞功能障碍有助于子痫前期的病理生理过程。
