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本文引用的文献

1
A Role for the Stele in Intertissue Signaling in the Initiation of Abscission in Bean Leaves (Phaseolus vulgaris L.).豆科植物(菜豆)叶片脱落起始过程中,中柱在组织间信号传导中的作用
Plant Physiol. 1994 May;105(1):341-347. doi: 10.1104/pp.105.1.341.
2
Pedicel breakstrength and cellulase gene expression during tomato flower abscission.番茄花脱落过程中的花梗断裂强度和纤维素酶基因表达
Plant Physiol. 1996 Jul;111(3):813-20. doi: 10.1104/pp.111.3.813.
3
Molecular characterization of one of the maize polygalacturonase gene family members which are expressed during late pollen development.在花粉发育后期表达的玉米多聚半乳糖醛酸酶基因家族成员之一的分子特征。
Plant J. 1993 Feb;3(2):261-71. doi: 10.1111/j.1365-313x.1993.tb00177.x.
4
Characterization of a tobacco gene encoding a pollen-specific polygalacturonase.一个编码花粉特异性多聚半乳糖醛酸酶的烟草基因的特性分析。
Plant Mol Biol. 1994 May;25(2):283-97. doi: 10.1007/BF00023244.
5
Two divergent endo-beta-1,4-glucanase genes exhibit overlapping expression in ripening fruit and abscising flowers.两个不同的内切β-1,4-葡聚糖酶基因在成熟果实和脱落花朵中表现出重叠表达。
Plant Cell. 1994 Oct;6(10):1485-93. doi: 10.1105/tpc.6.10.1485.
6
Patterns of amino acids near signal-sequence cleavage sites.信号序列切割位点附近的氨基酸模式。
Eur J Biochem. 1983 Jun 1;133(1):17-21. doi: 10.1111/j.1432-1033.1983.tb07424.x.
7
Characterization of a gene family abundantly expressed in Oenothera organensis pollen that shows sequence similarity to polygalacturonase.对在月见草花粉中大量表达且与多聚半乳糖醛酸酶具有序列相似性的一个基因家族的表征。
Plant Cell. 1990 Mar;2(3):263-74. doi: 10.1105/tpc.2.3.263.
8
Characterization of pollen polygalacturonase encoded by several cDNA clones in maize.玉米中几个cDNA克隆编码的花粉多聚半乳糖醛酸酶的特性分析
Plant Mol Biol. 1991 Dec;17(6):1155-64. doi: 10.1007/BF00028732.
9
Cellulase and polygalacturonase involvement in the abscission of leaf and fruit explants of peach.纤维素酶和多聚半乳糖醛酸酶与桃树叶和果实外植体脱落的关系
Plant Mol Biol. 1992 Dec;20(5):839-48. doi: 10.1007/BF00027155.

三种不同的多聚半乳糖醛酸酶在番茄叶片和花朵脱落过程中表达,每种酶都有不同的时间表达模式。

Three different polygalacturonases are expressed in tomato leaf and flower abscission, each with a different temporal expression pattern.

作者信息

Kalaitzis P, Solomos T, Tucker M L

机构信息

Plant Molecular Biology Laboratory, United States Department of Agriculture, Beltsville, Maryland 20705, USA.

出版信息

Plant Physiol. 1997 Apr;113(4):1303-8. doi: 10.1104/pp.113.4.1303.

DOI:10.1104/pp.113.4.1303
PMID:9112778
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC158253/
Abstract

Abscission, or organ separation, is accompanied by a marked increase in hydrolases, which are responsible for the degradation of the middle lamella and the loosening of the primary cell wall surrounding cells in the separation layer. We recently reported on the cloning of a tomato (Lycopersicon esculentum) polygalacturonase (PG) cDNA, TAPG1, expressed during leaf and flower abscission. In addition to TAPG1, we have cloned two more PG cDNAs (TAPG2 and TAPG4) that are also expressed during leaf and flower abscission. The peptide sequences for the three abscission PGs are relatively similar (76-93% identity) yet different from the those of tomato fruit PG (38-41% identity). None of the three abscission PG mRNAs are expressed in fruit, stems, petioles, or anthers of fully open flowers. An RNase protection assay revealed that all three PGs are expressed in leaf and flower abscission zones and in pistils of fully open flowers. TAPG4 mRNA is detected much earlier than TAPG1 and TAPG2 mRNA during both leaf and flower abscission.

摘要

脱落,即器官分离,伴随着水解酶的显著增加,这些水解酶负责中层薄片的降解以及分离层中围绕细胞的初生细胞壁的松弛。我们最近报道了一个在叶片和花朵脱落过程中表达的番茄(Lycopersicon esculentum)多聚半乳糖醛酸酶(PG)cDNA,即TAPG1的克隆。除了TAPG1,我们还克隆了另外两个也在叶片和花朵脱落过程中表达的PG cDNA(TAPG2和TAPG4)。这三种脱落相关PG的肽序列相对相似(同一性为76 - 93%),但与番茄果实PG的肽序列不同(同一性为38 - 41%)。这三种脱落相关PG的mRNA在果实、茎、叶柄或完全开放花朵的花药中均不表达。核糖核酸酶保护分析表明,所有这三种PG在叶片和花朵脱落区以及完全开放花朵的雌蕊中均有表达。在叶片和花朵脱落过程中,TAPG4 mRNA的检测时间比TAPG1和TAPG2 mRNA早得多。