鸡蛋卵转铁蛋白的等电分级分离
The isoelectric fractionation of hen's-egg ovotransferrin.
作者信息
Wenn R V, Williams J
出版信息
Biochem J. 1968 Jun;108(1):69-74. doi: 10.1042/bj1080069.
Abstract
- Hen ovotransferrin was examined by isoelectric fractionation. 2. The major component observed in starch-gel electrophoresis can be isolated from the minor component. 3. When non-saturating amounts of iron are added to ovotransferrin, isoelectric fractionation demonstrates the existence of three molecular species corresponding to the metal-free protein, the one-iron-atom-protein complex and the two-iron-atoms-protein complex. 4. Isoelectric fractionation of human serum labelled with (59)Fe suggests that the transferrin of normal human serum also exists as metal-free protein, the one-iron-atom-protein complex and the two-iron-atoms-protein complex. 5. It is concluded that the binding constants for the first and second iron atoms are similar.
摘要
- 通过等电分级法对鸡卵转铁蛋白进行了检测。2. 在淀粉凝胶电泳中观察到的主要成分可与次要成分分离。3. 当向卵转铁蛋白中添加非饱和量的铁时，等电分级法表明存在三种分子形式，分别对应无金属蛋白、一铁原子蛋白复合物和二铁原子蛋白复合物。4. 用(59)Fe标记的人血清的等电分级表明，正常人血清中的转铁蛋白也以无金属蛋白、一铁原子蛋白复合物和二铁原子蛋白复合物的形式存在。5. 得出的结论是，第一和第二个铁原子的结合常数相似。
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本文引用的文献
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Starch gel electrophoresis in a discontinous system of buffers.在不连续缓冲系统中的淀粉凝胶电泳。
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