Rao M N, Lakshman M R
Department of Medicine, George Washington University, Washington, DC, USA.
Alcohol Clin Exp Res. 1997 Apr;21(2):348-51.
We have previously demonstrated that chronic ethanol specifically decreases the hepatic level and rate of synthesis of 2,6-sialyltransferase (2,6-ST). To understand its mechanism of action, effects of 8 weeks of chronic ethanol feeding on the expression of sialyltransferase (ST) genes in rat liver and kidneys were determined by Northern-blot analysis of ST mRNAs. It was found that, compared with the pair-fed control rats, the percentage decreases in ST mRNAs in the ethanol-fed group were as follows: liver-Gal-beta-1,4GlcNAc alpha 2,6-ST (2,6-ST): 59% (p < 0.001); liver-Gal-beta-1,3GlcNAc alpha 2,3-ST (2,3-ST): 32% (p < 0.01); and kidneys-2,6-ST: 5% (NS). In contrast, glyceral-dehyde-3-phosphate dehydrogenase mRNA in both liver and kidneys was unaffected by the same ethanol treatment. Taken together, these results demonstrate that chronic ethanol downregulates the expression of 2,6-ST and 2,3-ST genes in rat liver.
我们先前已证明，慢性乙醇会特异性降低肝脏中2,6-唾液酸转移酶（2,6-ST）的水平及其合成速率。为了解其作用机制，通过对ST mRNA进行Northern印迹分析，确定了8周慢性乙醇喂养对大鼠肝脏和肾脏中唾液酸转移酶（ST）基因表达的影响。结果发现，与配对喂养的对照大鼠相比，乙醇喂养组中ST mRNA的降低百分比如下：肝脏-Gal-β-1,4GlcNAc α2,6-ST（2,6-ST）：59%（p < 0.001）；肝脏-Gal-β-1,3GlcNAc α2,3-ST（2,3-ST）：32%（p < 0.01）；肾脏-2,6-ST：5%（无显著性差异）。相比之下，相同乙醇处理对肝脏和肾脏中的甘油醛-3-磷酸脱氢酶mRNA均无影响。综上所述，这些结果表明慢性乙醇会下调大鼠肝脏中2,6-ST和2,3-ST基因的表达。
