Bessler H, Singer R, Raanani P, Levinsky H, Lahav M, Cohen A M
Research Institute, Hasharon Hospital Golda Medical Center, Petah Tiqva, Israel.
Biol Reprod. 1995 Dec;53(6):1474-7. doi: 10.1095/biolreprod53.6.1474.
Sperm surface glycoproteins are modified during passage through the epididymis, a process believed to be important in the production of functionally mature spermatozoa. The effect of various cytokines on reproductive events has recently been investigated, with conflicting results. In the present investigation, the effect of interferon-alpha-2b (IFN alpha 2b) on sialyltransferase (ST) activity and beta-galactoside alpha-2,6-sialyltransferase (Gal 2,6-ST) mRNA expression was studied in rat testicular tissue. The results revealed the presence of Gal 2,6-ST mRNA in rat testicular tissue, similar in molecular size to that found previously in rat spleen, lung, ovary, kidney, heart, and brain. In addition, we observed that IFN alpha 2b reduced Gal 2,6-ST mRNA and ST activity in rat testes by a comparable magnitude. These findings provide insight into an additional mechanism by which cytokines may affect the reproductive system.
精子表面糖蛋白在通过附睾的过程中会发生修饰，这一过程被认为对功能成熟精子的产生至关重要。最近人们研究了各种细胞因子对生殖事件的影响，但结果相互矛盾。在本研究中，我们在大鼠睾丸组织中研究了α-2b干扰素（IFNα2b）对唾液酸转移酶（ST）活性和β-半乳糖苷α-2,6-唾液酸转移酶（Gal 2,6-ST）mRNA表达的影响。结果显示，大鼠睾丸组织中存在Gal 2,6-ST mRNA，其分子大小与先前在大鼠脾脏、肺、卵巢、肾脏、心脏和大脑中发现的相似。此外，我们观察到IFNα2b使大鼠睾丸中Gal 2,6-ST mRNA和ST活性降低的幅度相当。这些发现为细胞因子可能影响生殖系统的另一种机制提供了见解。
