Resta R, Thompson L F
Oklahoma Medical Research Foundation, Immunobiology and Cancer Program, Oklahoma City, USA.
Cell Signal. 1997 Feb;9(2):131-9. doi: 10.1016/s0898-6568(96)00132-5.
CD73 (ecto-5'-nucleotidase), a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes, catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. The cellular distribution, cDNA sequence, and structure of CD73 are reviewed. CD73 serves as a costimulatory molecule in activating T cells. A Jurkat.T cell line transfected with the CD73 cDNA revealed that neither enzymatic activity nor the GPI anchor is necessary for T cell activation in vitro via CD73, while expression of p56kk, CD45 and the T cell receptor are required. Models for the transmission of signals via CD73 and other GPI-anchored proteins are discussed. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. The hypothesis that CD73 produces adenosine that is important for T cell development is presented.
CD73（胞外5'-核苷酸酶）是一种糖基磷脂酰肌醇（GPI）锚定的嘌呤补救酶，表达于人类T和B淋巴细胞表面，催化嘌呤和嘧啶核糖及脱氧核糖核苷单磷酸转化为相应的核苷。本文综述了CD73的细胞分布、cDNA序列及结构。CD73在激活T细胞过程中作为共刺激分子发挥作用。转染了CD73 cDNA的Jurkat.T细胞系显示，体外通过CD73激活T细胞既不需要酶活性也不需要GPI锚定，而p56kk、CD45和T细胞受体的表达是必需的。文中讨论了通过CD73和其他GPI锚定蛋白传递信号的模型。CD73产生的腺苷在许多生理系统的细胞信号传导中发挥作用，包括肠上皮、缺血心肌和胆碱能突触。本文提出了CD73产生对T细胞发育重要的腺苷这一假说。
