Dorr R A, Kierbel A, Vera J, Parisi M
Facultad de Medicina (UBA), Departamento de Fisiología, Buenos Aires, Argentina.
Comput Methods Programs Biomed. 1997 May;53(1):9-14. doi: 10.1016/s0169-2607(96)01801-9.
We describe a computer-based data-acquisition system designed for the measurement of net water flux across epithelia. It is based on the detection of a liquid meniscus position in a capillary tube using an electro-optical device. Then the data are digitized, transferred to the computer main memory and saved in a file. The advantages of the system are: (a) to measure net water transfer with an accuracy of 50 nl; (b) to switch from absorptive to secretory conditions in real time; (c) data are directly digitized to be analytically, graphically and statistically treated; (d) the specially developed software allows change of time intervals for data acquirement and the scale in graphic events; (e) the software also allows recall to the monitor screen of graphics from previous tests or to print them on paper; (f) a simple mechanical structure, saving maintenance and time; and (g) high reliability.
我们描述了一种基于计算机的数据采集系统，该系统设计用于测量上皮细胞的净水通量。它基于使用电光装置检测毛细管中液体弯月面的位置。然后数据被数字化，传输到计算机主存储器并保存在文件中。该系统的优点包括：(a) 以50 nl的精度测量净水转移；(b) 实时从吸收状态切换到分泌状态；(c) 数据直接数字化以便进行分析、图形和统计处理；(d) 专门开发的软件允许更改数据采集的时间间隔和图形事件的比例；(e) 该软件还允许从以前的测试中调出图形到监视器屏幕或打印在纸上；(f) 机械结构简单，节省维护和时间；以及(g) 高可靠性。
