DNA甲基化对组织转谷氨酰胺酶基因表达的调控。
Regulation of the expression of the tissue transglutaminase gene by DNA methylation.
作者信息
Lu S, Davies P J
机构信息
Department of Pharmacology, University of Texas Medical School, Houston, TX 77225, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4692-7. doi: 10.1073/pnas.94.9.4692.
Abstract
We have investigated the role of DNA methylation in the regulation of the expression of the human tissue transglutaminase gene. Studies on the methylation of the transglutaminase promoter in normal and neoplastic human cells demonstrated that the promoter is methylated in vivo and hypomethylation of the promoter is correlated with constitutive gene expression. Demethylation of the promoter in vivo by treatment of the cells with 5-azacytidine increased transglutaminase expression and hypermethylation of the promoter in vitro suppressed its activity. These studies suggest that alternations in DNA methylation may be one of the mechanisms regulating the tissue-specific expression of the tissue transglutaminase gene.
摘要
我们研究了DNA甲基化在人组织转谷氨酰胺酶基因表达调控中的作用。对正常和肿瘤人类细胞中转谷氨酰胺酶启动子甲基化的研究表明，该启动子在体内发生甲基化，且启动子的低甲基化与基因的组成型表达相关。用5-氮杂胞苷处理细胞使启动子在体内发生去甲基化，从而增加了转谷氨酰胺酶的表达，而启动子在体外的高甲基化则抑制了其活性。这些研究表明，DNA甲基化的改变可能是调节组织转谷氨酰胺酶基因组织特异性表达的机制之一。
相似文献
1
Regulation of the expression of the tissue transglutaminase gene by DNA methylation.DNA甲基化对组织转谷氨酰胺酶基因表达的调控。
Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4692-7. doi: 10.1073/pnas.94.9.4692.
2
Identification of a transforming growth factor-beta1/bone morphogenetic protein 4 (TGF-beta1/BMP4) response element within the mouse tissue transglutaminase gene promoter.小鼠组织转谷氨酰胺酶基因启动子中转化生长因子-β1/骨形态发生蛋白4（TGF-β1/BMP4）反应元件的鉴定。
J Biol Chem. 1998 May 22;273(21):12798-806. doi: 10.1074/jbc.273.21.12798.
3
Specific methylation of the CpG-rich domains in the promoter of the human tissue transglutaminase gene.人组织转谷氨酰胺酶基因启动子中富含CpG结构域的特异性甲基化
Gene. 2002 Sep 4;297(1-2):103-12. doi: 10.1016/s0378-1119(02)00874-0.
4
Mapping and sequencing of the murine 'tissue' transglutaminase (Tgm2) gene: absence of mutations in MRLlpr/lpr mice.小鼠“组织”转谷氨酰胺酶（Tgm2）基因的定位与测序：MRLlpr/lpr小鼠中无突变
Cell Death Differ. 1999 Mar;6(3):216-7. doi: 10.1038/sj.cdd.4400494.
5
The interplay of ubiquitous DNA-binding factors, availability of binding sites in the chromatin, and DNA methylation in the differential regulation of phosphoenolpyruvate carboxykinase gene expression.
Nucleic Acids Res. 1991 Sep 11;19(17):4681-8. doi: 10.1093/nar/19.17.4681.
6
Cardiac specific overexpression of transglutaminase II (G(h)) results in a unique hypertrophy phenotype independent of phospholipase C activation.转谷氨酰胺酶II（G(h)）在心脏中的特异性过表达会导致一种独特的肥大表型，且不依赖于磷脂酶C的激活。
J Biol Chem. 1999 Jul 23;274(30):21291-6. doi: 10.1074/jbc.274.30.21291.
7
Identification and functional characterization of the murine Rac2 gene promoter.小鼠Rac2基因启动子的鉴定及功能特性分析
DNA Cell Biol. 1999 Mar;18(3):253-63. doi: 10.1089/104454999315475.
8
Colocalization of tissue transglutaminase and stress fibers in human vascular smooth muscle cells and human umbilical vein endothelial cells.组织转谷氨酰胺酶与应力纤维在人血管平滑肌细胞和人脐静脉内皮细胞中的共定位
Exp Cell Res. 1997 Feb 25;231(1):38-49. doi: 10.1006/excr.1996.3448.
9
The human prostate-specific transglutaminase gene (TGM4): genomic organization, tissue-specific expression, and promoter characterization.人类前列腺特异性转谷氨酰胺酶基因（TGM4）：基因组结构、组织特异性表达及启动子特征分析
Genomics. 1998 Aug 1;51(3):434-44. doi: 10.1006/geno.1998.5393.
引用本文的文献
1
Role of epigenetics in corneal health and disease.表观遗传学在角膜健康与疾病中的作用。
Prog Retin Eye Res. 2025 Jan;104:101318. doi: 10.1016/j.preteyeres.2024.101318. Epub 2024 Nov 14.
2
Prognostic significance of factor XIIIA promoter methylation status in aneurysmal subarachnoid haemorrhage (aSAH).因子 XIIIa 启动子甲基化状态对颅内动脉瘤性蛛网膜下腔出血（aSAH）的预后意义。
BMC Cardiovasc Disord. 2019 Jul 17;19(1):170. doi: 10.1186/s12872-019-1146-8.
3
Epigenetics and inheritance of phenotype variation in livestock.家畜表型变异的表观遗传学与遗传
Epigenetics Chromatin. 2016 Jul 21;9:31. doi: 10.1186/s13072-016-0081-5. eCollection 2016.
4
Cellular functions of tissue transglutaminase.组织转谷氨酰胺酶的细胞功能。
Int Rev Cell Mol Biol. 2012;294:1-97. doi: 10.1016/B978-0-12-394305-7.00001-X.
5
Aberrant DNA methylation of matrix remodeling and cell adhesion related genes in pterygium.翼状胬肉中基质重塑和细胞黏附相关基因的异常 DNA 甲基化。
PLoS One. 2011 Feb 16;6(2):e14687. doi: 10.1371/journal.pone.0014687.
6
7
Folate regulation of axonal regeneration in the rodent central nervous system through DNA methylation.通过 DNA 甲基化调节啮齿动物中枢神经系统中的轴突再生。
J Clin Invest. 2010 May;120(5):1603-16. doi: 10.1172/JCI40000. Epub 2010 Apr 26.
8
Elimination of Colon Cancer Stem-Like Cells by the Combination of Curcumin and FOLFOX.姜黄素和 FOLFOX 联合消除结肠癌干细胞样细胞。
Transl Oncol. 2009 Dec;2(4):321-8. doi: 10.1593/tlo.09193.
9
Transglutaminase 2 as a cisplatin resistance marker in non-small cell lung cancer.转谷氨酰胺酶 2 作为非小细胞肺癌顺铂耐药标志物。
J Cancer Res Clin Oncol. 2010 Apr;136(4):493-502. doi: 10.1007/s00432-009-0681-6. Epub 2009 Sep 18.
10
Identification of two GTP-independent alternatively spliced forms of tissue transglutaminase in human leukocytes, vascular smooth muscle, and endothelial cells.在人白细胞、血管平滑肌和内皮细胞中鉴定出两种不依赖鸟苷三磷酸（GTP）的组织转谷氨酰胺酶可变剪接形式。
FASEB J. 2007 Dec;21(14):4131-43. doi: 10.1096/fj.06-7598com. Epub 2007 Jul 3.
本文引用的文献
1
Identification and characterization of a versatile retinoid response element (retinoic acid receptor response element-retinoid X receptor response element) in the mouse tissue transglutaminase gene promoter.小鼠组织转谷氨酰胺酶基因启动子中多功能视黄酸反应元件（视黄酸受体反应元件-视黄酸X受体反应元件）的鉴定与表征
J Biol Chem. 1996 Feb 23;271(8):4355-65. doi: 10.1074/jbc.271.8.4355.
2
Expression of prokaryotic HhaI DNA methyltransferase is transforming and lethal to NIH 3T3 cells.原核生物HhaI DNA甲基转移酶的表达对NIH 3T3细胞具有转化作用且具有致死性。
Cancer Res. 1996 Feb 1;56(3):616-22.
3
Nucleosome displacement in transcription.转录过程中的核小体移位
Cell. 1993 Feb 12;72(3):305-8. doi: 10.1016/0092-8674(93)90109-4.
4
Site-specific methylation inhibits transcriptional activity of platelet-derived growth factor A-chain promoter.位点特异性甲基化抑制血小板衍生生长因子A链启动子的转录活性。
J Biol Chem. 1993 Aug 15;268(23):17334-40.
5
Activity and distribution of tissue transglutaminase in association with nerve-muscle synapses.组织转谷氨酰胺酶与神经肌肉突触相关的活性及分布
J Neurochem. 1993 Sep;61(3):1064-72. doi: 10.1111/j.1471-4159.1993.tb03621.x.
6
Expression of tissue transglutaminase in skeletal tissues correlates with events of terminal differentiation of chondrocytes.组织转谷氨酰胺酶在骨骼组织中的表达与软骨细胞终末分化事件相关。
J Cell Biol. 1993 Mar;120(6):1461-70. doi: 10.1083/jcb.120.6.1461.
7
Identification of promoter region of guinea pig liver transglutaminase gene.豚鼠肝脏转谷氨酰胺酶基因启动子区域的鉴定
Biochim Biophys Acta. 1993 Mar 20;1172(3):319-22. doi: 10.1016/0167-4781(93)90221-x.
8
Progressive increases in the methylation status and heterochromatinization of the myoD CpG island during oncogenic transformation.在致癌转化过程中，肌分化抗原（MyoD）基因座控制区（CpG岛）的甲基化状态和异染色质化逐渐增加。
Mol Cell Biol. 1994 Sep;14(9):6143-52. doi: 10.1128/mcb.14.9.6143-6152.1994.
9
Role of DNA methylation in the regulation of transcription.DNA甲基化在转录调控中的作用。
Curr Opin Genet Dev. 1994 Apr;4(2):255-9. doi: 10.1016/s0959-437x(05)80052-8.
10
Tissue transglutaminase and apoptosis: sense and antisense transfection studies with human neuroblastoma cells.组织转谷氨酰胺酶与细胞凋亡：用人神经母细胞瘤细胞进行的正反义转染研究
Mol Cell Biol. 1994 Oct;14(10):6584-96. doi: 10.1128/mcb.14.10.6584-6596.1994.
Zotero 插件