用于鉴定纽约市耐利福平结核分枝杆菌分离株中rpoB基因突变的线性探针检测试剂盒的评估
Evaluation of a line probe assay kit for characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from New York City.
作者信息
Cooksey R C, Morlock G P, Glickman S, Crawford J T
机构信息
Division of AIDS, Sexually Transmitted Diseases, and Tuberculosis Laboratory Research, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 1997 May;35(5):1281-3. doi: 10.1128/jcm.35.5.1281-1283.1997.
Abstract
A commercial line probe assay kit (Inno-LiPA Rif.TB) for rapid identification of mutations in the rpoB gene associated with rifampin resistance in Mycobacterium tuberculosis was evaluated with a collection of 51 rifampin-resistant strains. Nine distinct rpoB mutations were identified. Concordances with automated sequence results for five wild-type kit probes and four probes for specific mutations were 94.1 and 100%, respectively. Overall concordance of the line probe assay kit with phenotypic rifampin susceptibility testing results was 90.2%.
摘要
使用一套51株耐利福平菌株对一种用于快速鉴定结核分枝杆菌中与利福平耐药相关的rpoB基因突变的商业线性探针检测试剂盒（Inno-LiPA Rif.TB）进行了评估。鉴定出9种不同的rpoB突变。5种野生型试剂盒探针和4种特定突变探针与自动测序结果的一致性分别为94.1%和100%。该线性探针检测试剂盒与利福平表型药敏试验结果的总体一致性为90.2%。
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