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Sequence-specific recognition of cytosine C5 and adenine N6 DNA methyltransferases requires different deformations of DNA.胞嘧啶C5和腺嘌呤N6 DNA甲基转移酶的序列特异性识别需要DNA的不同变形。
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Determination of the DNA bend angle induced by the restriction endonuclease EcoRV in the presence of Mg2+.在Mg2+存在的情况下，测定限制性内切酶EcoRV诱导的DNA弯曲角度。
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Crystal structure of the HhaI DNA methyltransferase complexed with S-adenosyl-L-methionine.与S-腺苷-L-甲硫氨酸复合的HhaI DNA甲基转移酶的晶体结构。
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HhaI methyltransferase flips its target base out of the DNA helix.HhaI甲基转移酶将其靶碱基翻转出DNA螺旋。
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The DNA (cytosine-5) methyltransferases.DNA（胞嘧啶-5）甲基转移酶
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HMG domain proteins induce sharp bends in cisplatin-modified DNA.HMG结构域蛋白可诱导顺铂修饰的DNA产生急剧弯曲。
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DNA-bend modulation in a repressor-to-activator switching mechanism.阻遏物到激活物转换机制中的DNA弯曲调节
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Pre-bending of a promoter sequence enhances affinity for the TATA-binding factor.启动子序列的预弯曲可增强对TATA结合因子的亲和力。
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9
The CpG-specific methylase SssI has topoisomerase activity in the presence of Mg2+.CpG特异性甲基化酶SssI在Mg2+存在的情况下具有拓扑异构酶活性。
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The crystal structure of HaeIII methyltransferase convalently complexed to DNA: an extrahelical cytosine and rearranged base pairing.与DNA共价结合的HaeIII甲基转移酶的晶体结构：一个螺旋外胞嘧啶和重排的碱基配对。
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MspI DNA甲基转移酶与DNA形成序列特异性复合物时所诱导的弯曲角度和弯曲轨迹。

Angle and locus of the bend induced by the msp I DNA methyltransferase in a sequence-specific complex with DNA.

作者信息

Dubey A K, Bhattacharya S K

机构信息

Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology-Delhi, Hauz Khas, New Delhi -110016, India.

出版信息

Nucleic Acids Res. 1997 May 15;25(10):2025-9. doi: 10.1093/nar/25.10.2025.

DOI:10.1093/nar/25.10.2025
PMID:9115372
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146686/
Abstract

Bending of DNA induced by M.Msp I, one of the m5C-DNA methyltransferases, has been investigated using circular permutation analysis. The M.Msp I MTase induced sharp bends in DNA containing its recognition sequence 5'-CCGG-3'which was estimated to be 142+/-4 degrees and 132+/-4 degrees for circularly permuted DNA fragments of 127 and 1459 bp respectively. The bend centre was found to be asymmetric with respect to the CCGG sequence and appeared to exclude the 'target cytosine'. An estimate of approximately 15 kcal/mol was obtained for the free energy associated with M.Msp I-induced DNA bending.

摘要

利用环形置换分析研究了m5C - DNA甲基转移酶之一的M.Msp I诱导的DNA弯曲。M.Msp I甲基转移酶在含有其识别序列5'-CCGG-3'的DNA中诱导了急剧弯曲，对于127 bp和1459 bp的环形置换DNA片段，估计弯曲角度分别为142±4度和132±4度。发现弯曲中心相对于CCGG序列是不对称的，并且似乎排除了“目标胞嘧啶”。获得了与M.Msp I诱导的DNA弯曲相关的自由能约为15 kcal/mol的估计值。