MspI DNA甲基转移酶与DNA形成序列特异性复合物时所诱导的弯曲角度和弯曲轨迹。
Angle and locus of the bend induced by the msp I DNA methyltransferase in a sequence-specific complex with DNA.
作者信息
Dubey A K, Bhattacharya S K
机构信息
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology-Delhi, Hauz Khas, New Delhi -110016, India.
出版信息
Nucleic Acids Res. 1997 May 15;25(10):2025-9. doi: 10.1093/nar/25.10.2025.
Abstract
Bending of DNA induced by M.Msp I, one of the m5C-DNA methyltransferases, has been investigated using circular permutation analysis. The M.Msp I MTase induced sharp bends in DNA containing its recognition sequence 5'-CCGG-3'which was estimated to be 142+/-4 degrees and 132+/-4 degrees for circularly permuted DNA fragments of 127 and 1459 bp respectively. The bend centre was found to be asymmetric with respect to the CCGG sequence and appeared to exclude the 'target cytosine'. An estimate of approximately 15 kcal/mol was obtained for the free energy associated with M.Msp I-induced DNA bending.
摘要
利用环形置换分析研究了m5C - DNA甲基转移酶之一的M.Msp I诱导的DNA弯曲。M.Msp I甲基转移酶在含有其识别序列5'-CCGG-3'的DNA中诱导了急剧弯曲，对于127 bp和1459 bp的环形置换DNA片段，估计弯曲角度分别为142±4度和132±4度。发现弯曲中心相对于CCGG序列是不对称的，并且似乎排除了“目标胞嘧啶”。获得了与M.Msp I诱导的DNA弯曲相关的自由能约为15 kcal/mol的估计值。
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2
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3
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