酵母菌落大小反映酵母人工染色体（YAC）的拷贝数。
Yeast colony size reflects YAC copy number.
作者信息
Popov A V, Bützler C, Brüggemann M
机构信息
Department of Development and Genetics, The Babraham Institute, Cambridge CB2 4AT, UK.
出版信息
Nucleic Acids Res. 1997 May 15;25(10):2039-40. doi: 10.1093/nar/25.10.2039.
Abstract
A novel strategy for separation of co-cloned YACs was developed. For this, yeast cells were grown under non-selective conditions to allow the mitotic loss of multiple YACs. Yeast colonies of different size appear on 'drop-out' selection plates with small clones consistently containing a single-copy YAC. Different auxotrophic marker genes can be used to separate co-cloned YACs or reduce their copy number, which is essential for most YAC-modification procedures.
摘要
开发了一种分离共克隆酵母人工染色体（YAC）的新策略。为此，酵母细胞在非选择性条件下生长，以使多个YAC发生有丝分裂丢失。在“缺陷型”选择平板上出现不同大小的酵母菌落，小克隆始终包含单拷贝YAC。不同的营养缺陷型标记基因可用于分离共克隆的YAC或减少其拷贝数，这对于大多数YAC修饰程序至关重要。
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