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Targeted alterations in yeast artificial chromosomes for inter-species gene transfer.

作者信息

Davies N P, Rosewell I R, Brüggemann M

机构信息

AFRC, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.

出版信息

Nucleic Acids Res. 1992 Jun 11;20(11):2693-8. doi: 10.1093/nar/20.11.2693.

DOI:10.1093/nar/20.11.2693
PMID:1614855
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336909/
Abstract

In order to facilitate alterations of large DNA molecules for their introduction into mammalian cells we have characterised the mechanism of site-specific modifications in yeast artificial chromosomes (YACs). Newly developed yeast integration vectors with dominant selectable marker genes allow targeted integration into left (centromeric) and right (non-centromeric) YAC arms as well as alterations to the human derived insert DNA. In transformation experiments, integration proceeds exclusively by homologous recombination although yeast prefers linear ends of homology for predefined insertions. Targeted regions can be rescued which expedite the cloning of internal human sequences and the identification of 5' and 3' YAC/insert borders. Integration of the neomycin resistance gene into various parts of the YAC allowed the transfer and stable integration of large DNA molecules into a variety of mammalian cells including embryonic stem cells.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/89eb2806cc64/nar00085-0077-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/63bf75d0058a/nar00085-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/ed9cd05e8027/nar00085-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/7476edf74bca/nar00085-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/0054e9cf87e9/nar00085-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/89eb2806cc64/nar00085-0077-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/63bf75d0058a/nar00085-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/ed9cd05e8027/nar00085-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/7476edf74bca/nar00085-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/0054e9cf87e9/nar00085-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a83f/336909/89eb2806cc64/nar00085-0077-b.jpg

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High-affinity IgG antibodies develop naturally in Ig-knockout rats carrying germline human IgH/Igκ/Igλ loci bearing the rat CH region.高亲和力 IgG 抗体在携带胚系人 IgH/Igκ/Igλ 基因座并带有大鼠 CH 区的 Ig 敲除大鼠中自然产生。

本文引用的文献

1
Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments.克隆的人类和小鼠κ免疫球蛋白恒定区和J区基因在功能片段上保持同源性。
Cell. 1980 Nov;22(1 Pt 1):197-207. doi: 10.1016/0092-8674(80)90168-3.
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Genomic sequencing.基因组测序
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Yeast colony size reflects YAC copy number.酵母菌落大小反映酵母人工染色体（YAC）的拷贝数。
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Nucleic Acids Res. 1996 Jun 15;24(12):2271-80. doi: 10.1093/nar/24.12.2271.
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Extension of yeast artificial chromosomes by cosmid multimers.通过黏粒多聚体扩展酵母人工染色体
Nucleic Acids Res. 1993 Feb 11;21(3):767-8. doi: 10.1093/nar/21.3.767.
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
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Base sequence studies of 300 nucleotide renatured repeated human DNA clones.300个核苷酸的复性重复人类DNA克隆的碱基序列研究。
J Mol Biol. 1981 Sep 5;151(1):17-33. doi: 10.1016/0022-2836(81)90219-9.
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Yeast transformation: a model system for the study of recombination.酵母转化：用于重组研究的模型系统。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8. doi: 10.1073/pnas.78.10.6354.
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Structure and function of the yeast URA3 gene: expression in Escherichia coli.酵母URA3基因的结构与功能：在大肠杆菌中的表达
Gene. 1984 Jul-Aug;29(1-2):113-24. doi: 10.1016/0378-1119(84)90172-0.
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Construction of LYS2 cartridges for use in genetic manipulations of Saccharomyces cerevisiae.用于酿酒酵母基因操作的LYS2盒的构建。
Gene. 1986;46(2-3):237-45. doi: 10.1016/0378-1119(86)90408-7.
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Transformation of yeast spheroplasts without cell fusion.无细胞融合的酵母原生质体转化
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