Daniels J T, Kearney J N, Ingham E
Yorkshire Regional Tissue Bank, Pinderfields General Hospital, Wakefield, UK.
Burns. 1997 Feb;23(1):26-31. doi: 10.1016/s0305-4179(96)00061-7.
The purpose of this investigation was to assess the potential of commercially available extracellular matrix proteins, as enhancers of human keratinocyte attachment and proliferation, with a view to their incorporation into a skin equivalent. The following substrates were studied: type I and type IV collagen, fibronectin, gelatin and laminin. Human keratinocytes were cultured in low-calcium, serum-free medium. The number of cells attached to each substrate, observed under phase-contrast inverted microscopy in randomly selected fields of view, were counted 2 h afterseeding. Measurements of growth rate and colony-forming efficiency were made at 24-h intervals. None of the substrates tested were found to have an effect significant enough to warrant further investigation or inclusion into skin equivalent.
本研究的目的是评估市售细胞外基质蛋白作为人角质形成细胞附着和增殖增强剂的潜力，以期将其整合到皮肤替代物中。研究了以下底物：I型和IV型胶原、纤连蛋白、明胶和层粘连蛋白。人角质形成细胞在低钙无血清培养基中培养。接种2小时后，在相差倒置显微镜下随机选择视野观察附着在每个底物上的细胞数量并进行计数。每隔24小时测量生长速率和集落形成效率。所测试的底物均未发现有足够显著的效果，值得进一步研究或将其纳入皮肤替代物。
