Kainulainen T, Autio-Harmainen H, Oikarinen A, Salo S, Tryggvason K, Salo T
Oral and Maxillofacial Department, Oulu University Hospital, Finland.
Br J Dermatol. 1997 Mar;136(3):331-6.
Laminin-5 is a glycoprotein which mediates epithelial cell adhesion to the basement membrane. This study describes the distribution and synthesis of laminin-5 in oral lichen planus, epithelial dysplasias, squamous cell carcinomas and a lymph node metastasis using immunohistochemistry and in situ hybridization. In normal oral mucosa and lichen planus, immunoreaction to the laminin-5 was seen as a thin continuous, delicate line in the basement membrane region, although slight irregularities in the thickness and intensity of the immunoreaction could be detected in some cases with lichen planus. In epithelial dysplasias, the laminin-5 staining was discontinuous and more diffuse compared to lichen planus and normal mucosa. The immunoreaction was generally extracellular, although in some cases with lichen planus and epithelial dysplasia there were a few basal epithelial cells showing cytoplasmic staining. The invasive carcinomas and the lymph node metastasis showed a striking, intense cytoplasmic, staining of the carcinoma cells along the invasive border of the neoplastic islands and in individual infiltrating carcinoma cells. Using in situ hybridization, the laminin-5 gamma 2 chain mRNA expression could not be detected in normal oral mucosa whereas, in non-dysplastic lichen planus and, more strongly, in dysplasias, there was a clear increase in the expression of laminin-5 mRNA in the basal epithelial cells. The most intensive signal was detected in the invasive front of the oral squamous cell carcinomas and the lymph node metastasis. We conclude that, in oral squamous cell carcinoma, there is altered synthesis and secretion of laminin-5 mRNA and protein. It is also evident that in dysplastic lesions of oral epithelium the synthesis and distribution of laminin-5 is abnormal.
层粘连蛋白-5是一种介导上皮细胞与基底膜黏附的糖蛋白。本研究采用免疫组织化学和原位杂交技术，描述了层粘连蛋白-5在口腔扁平苔藓、上皮发育异常、鳞状细胞癌及淋巴结转移灶中的分布与合成情况。在正常口腔黏膜和扁平苔藓中，层粘连蛋白-5的免疫反应在基底膜区域表现为一条细的连续、精致的线，尽管在某些扁平苔藓病例中可检测到免疫反应的厚度和强度存在轻微不规则。在上皮发育异常中，与扁平苔藓和正常黏膜相比，层粘连蛋白-5染色不连续且更弥漫。免疫反应一般位于细胞外，尽管在某些扁平苔藓和上皮发育异常病例中，有一些基底上皮细胞显示胞质染色。浸润性癌和淋巴结转移灶显示，在肿瘤岛的浸润边缘及单个浸润癌细胞中，癌细胞有显著、强烈的胞质染色。采用原位杂交技术，在正常口腔黏膜中未检测到层粘连蛋白-5γ2链mRNA表达，而在非发育异常的扁平苔藓中，以及在发育异常中更明显的是，基底上皮细胞中层粘连蛋白-5 mRNA表达明显增加。在口腔鳞状细胞癌和淋巴结转移灶的浸润前沿检测到最强的信号。我们得出结论，在口腔鳞状细胞癌中，层粘连蛋白-5 mRNA和蛋白的合成与分泌发生了改变。同样明显的是，在口腔上皮发育异常病变中，层粘连蛋白-5的合成与分布是异常的。
