Mielcarek-Kuchta Daniela, Olofsson Jan, Golusinski Wojciech
Przybyszewskiego Street 49, 60-355 Poznan, Poland.
Eur Arch Otorhinolaryngol. 2008 Oct;265(10):1257-61. doi: 10.1007/s00405-008-0666-0. Epub 2008 May 31.
Laminins are a family of glycoproteins that consist of one heavy alpha chain and two light beta and gamma chains. The laminin molecule is a major component of the basement membrane and plays an important roles in cell differentiation, adhesion, and migration. It has been reported that laminin is strongly expressed at the invasive front of the tumor and plays an important role in forming the metastases. The aim of the study was to assess the importance of laminin expression in a group of patients with laryngeal carcinoma and neck metastases. The study was carried out on 70 patients with squamous cell carcinoma of the larynx treated at the ENT Department University of Medical Sciences in Poznań. The clinical data consisted of sex, age, stage of the tumor, and histological and immunohistochemical studies. The average time of the follow-up was 3 years. The immunohistochemical study was performed using formalin-fixed and paraffin-embedded tissue sections. Monoclonal mouse anti-human laminin (DAKO, code no. MO638) antibodies were used for immunohistochemistry. To evaluate the laminin staining, a four-point grading system were used: score 0, continuous; score 1, loss of staining <10%; score 2, loss of staining <50%; score 3, loss of staining >50%. The correlation between staining intensity of the laminin and the presence of neck metastases (classified as N-positive "N+" and N-negative "N-"neck) was calculated. All parameters underwent statistical analysis. The patients with advanced clinical disease dominated in our material. Most of the tumors were classified as T3 and T4. A total of 36 patients had metastases to the regional lymph nodes. Positive laminin staining was observed in 62 cases. There was no correlation between laminin staining and occurrence of neck metastases in our studied group (30 N+ and 32 N-). The gravity level in Wilcoxon test was at P < 0.000001 and in Spearman rank order correlation was statistically not significant (P > 0.09).
层粘连蛋白是一类糖蛋白，由一条重的α链和两条轻的β链及γ链组成。层粘连蛋白分子是基底膜的主要成分，在细胞分化、黏附和迁移中起重要作用。据报道，层粘连蛋白在肿瘤侵袭前沿强烈表达，并在转移形成中起重要作用。本研究的目的是评估层粘连蛋白表达在一组喉癌伴颈部转移患者中的重要性。该研究对70例在波兹南医科大学耳鼻喉科接受治疗的喉鳞状细胞癌患者进行。临床资料包括性别、年龄、肿瘤分期以及组织学和免疫组织化学研究。平均随访时间为3年。免疫组织化学研究使用福尔马林固定、石蜡包埋的组织切片。单克隆小鼠抗人层粘连蛋白（DAKO，编号MO638）抗体用于免疫组织化学。为评估层粘连蛋白染色，采用四点分级系统：0分，连续染色；1分，染色缺失<10%；2分，染色缺失<50%；3分，染色缺失>50%。计算层粘连蛋白染色强度与颈部转移（分为N阳性“N+”和N阴性“N-”颈部）的相关性。所有参数均进行统计分析。我们的研究材料中以临床疾病晚期的患者为主。大多数肿瘤分类为T3和T4。共有36例患者出现区域淋巴结转移。62例观察到层粘连蛋白染色阳性。在我们的研究组（30例N+和32例N-）中，层粘连蛋白染色与颈部转移的发生之间没有相关性。Wilcoxon检验的显著性水平为P<0.000001，Spearman等级相关性在统计学上不显著（P>0.09）。
