Turlin E, Gasser F, Biville F
Département de Biochimie et Génétique Moléculaire, Institut Pasteur, Paris, France.
Biochimie. 1996;78(10):823-31. doi: 10.1016/s0300-9084(97)84334-9.
A 7361 kb fragment of E coli chromosomal DNA able to complement pqqE and pqqF mutants of Methylobacterium organophilum has been sequenced. Five open reading frames (ORF) have been identified. Four ORFs (102, 103, 106 and 107), belong to a single transcription unit. They are separated by a transcription termination site from a fifth ORF (ORF109). Polypeptides of 28, 85 and 82 kDa encoded by ORFs 102, 103 and 106 respectively were visualised in maxi-cell experiments. Both ORF106 and ORF107 are required for complementations of pqqE and pqqF mutants from M organophilum. The polypeptides encoded by ORFs102, 103 and 107 have no homologies with the products of pqq genes previously sequenced from Acinetobacter calcoaceticus, Klebsiella pneumoniae, and Methylobacterium extorquens AM1. The polypeptide encoded by ORF106 shows homology with the pqqF gene product of K pneumoniae, and seems to belong to a family of zinc proteases. The sequence of ORF109 is identical to the sequence of the gadB gene of E coli encoding for a glutamate decarboxylase.
已对一段7361 kb的大肠杆菌染色体DNA片段进行了测序，该片段能够互补嗜有机甲基杆菌的pqqE和pqqF突变体。已鉴定出五个开放阅读框（ORF）。四个ORF（102、103、106和107）属于单个转录单元。它们被一个转录终止位点与第五个ORF（ORF109）隔开。在大细胞实验中观察到了分别由ORF102、103和106编码的28 kDa、85 kDa和82 kDa的多肽。ORF106和ORF107都是互补嗜有机甲基杆菌的pqqE和pqqF突变体所必需的。由ORF102、103和107编码的多肽与先前从醋酸钙不动杆菌、肺炎克雷伯菌和嗜甲基营养菌AM1测序的pqq基因产物没有同源性。由ORF106编码的多肽与肺炎克雷伯菌的pqqF基因产物显示出同源性，似乎属于锌蛋白酶家族。ORF109的序列与编码谷氨酸脱羧酶的大肠杆菌gadB基因的序列相同。
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