Schulz S, Schreff M, Nüss D, Gramsch C, Höllt V
Department of Pharmacology and Toxicology, Otto-von-Guericke-University, Magdeburg, Germany.
Neuroreport. 1996 Nov 25;7(18):3021-5. doi: 10.1097/00001756-199611250-00045.
Antisera were generated against nociceptin/orphanin FQ, the putative ligand of the opioid receptor-like ORL1 receptor. Dot blot analysis showed that the antibodies selectively detect nociceptin but not dynorphin or other opioid peptides. Immunofluorescent staining of tissue sections revealed dense plexus of nociceptin-immunoreactive nerve fibres and terminals within the spinal cord dorsal horn, sensory trigeminal complex, raphe nuclei, locus coeruleus, periaqueductal grey, amygdala, habenula, hypothalamic region and septal area in mice and rats. When adjacent sections were stained either with the nociceptin antibody or the pan-opioid 3-E7 mouse monoclonal antibody, an overlapping distribution was observed in many nociceptive centres including the superficial dorsal horn, sensory trigeminal complex and periaqueductal grey. However, confocal microscopic examination of dual-labelled spinal cord and brain stem sections showed no instances of co-localization of nociceptin and opioid peptides in these regions. Intracerebroventricular administration of nociceptin has been shown to induce hyperalgesia. Thus, the present results suggest that nociceptin and opioids are released from different terminals thereby modulating pain signals in opposite ways.
制备了针对痛敏肽/孤啡肽FQ（阿片样物质受体样ORL1受体的假定配体）的抗血清。斑点印迹分析表明，这些抗体能选择性地检测痛敏肽，而不能检测强啡肽或其他阿片肽。组织切片的免疫荧光染色显示，在小鼠和大鼠的脊髓背角、感觉三叉神经复合体、中缝核、蓝斑、导水管周围灰质、杏仁核、缰核、下丘脑区域和隔区，存在密集的痛敏肽免疫反应性神经纤维和终末丛。当相邻切片用痛敏肽抗体或泛阿片样物质3-E7小鼠单克隆抗体染色时，在许多伤害性感受中枢，包括脊髓背角浅层、感觉三叉神经复合体和导水管周围灰质，观察到重叠分布。然而，对脊髓和脑干双重标记切片的共聚焦显微镜检查显示，在这些区域没有痛敏肽和阿片肽共定位的情况。脑室内注射痛敏肽已被证明可诱导痛觉过敏。因此，目前的结果表明，痛敏肽和阿片样物质从不同的终末释放，从而以相反的方式调节疼痛信号。
