Florin S, Meunier J, Costentin J
Unité de Neuropsychopharmacologie expérimentale (CNRS UMR 6036), Institut Fédératif de Recherche Multidisciplinaire des Peptides (IFRMP 23), Faculté de Médecine et de Pharmacie de Rouen, 22 Boulevard Gambetta, 76183, Rouen, Cedex, Franc.
Brain Res. 2000 Oct 13;880(1-2):11-6. doi: 10.1016/s0006-8993(00)02669-x.
The binding sites of nociceptin (also named orphanin FQ), the endogenous ligand of ORL1 (opiate receptor like 1), were localized in rat brain, using an autoradiographic procedure. High levels of binding were observed in the cingulate, retrosplenial, perirhinal, insular and occipital cortex, anterior and posteromedial cortical amygdaloid nuclei, basolateral amygdaloid nucleus, amygdaloid complex, posterior hippocampus, dorsal endopiriform, central medial thalamic, paraventricular, rhomboid thalamic, suprachiasmatic, ventromedial hypothalamic nuclei, mammillary complex, superficial gray layer of the superior colliculus, locus coeruleus, dorsal raphe nucleus. More moderate labelling was observed in the prefrontal, fronto-parietal, temporal, piriform cortex, dentate gyrus, anterior olfactory nucleus, olfactory tubercle, shell of nucleus accumbens, claustrum, lateral septum, laterodorsal thalamic, medial habenular, subthalamic, reuniens thalamic nuclei, subiculum, periaqueductal grey matter and pons. A lower binding site density was observed in the anterior and medial hippocampus, olfactory bulb, caudate putamen, the core of the nucleus accumbens, medial septum, ventrolateral, ventroposterolateral and mediodorsal thalamic nuclei, lateral and medial geniculate nuclei, hypothalamic area, substantia nigra, ventral tegmentum area and interpedoncular nucleus. A moderate and similar labelling was found in the dorsal and ventral horn of the spinal cord. No labelling was apparent in the corpus callosum. Thus, it appears that the ORL1 receptor is particularly abundant in the cerebral cortex, limbic system of the rat brain and some areas involved in pain perception.
采用放射自显影技术，在大鼠脑中定位了痛敏肽（也称为孤啡肽FQ）的结合位点，它是阿片受体样1（ORL1）的内源性配体。在扣带回、压后皮质、嗅周皮质、脑岛皮质和枕叶皮质、杏仁前核和杏仁后内侧皮质核、杏仁基底外侧核、杏仁复合体、海马后部、背侧内梨状区、丘脑中央内侧核、室旁核、菱形丘脑核、视交叉上核、下丘脑腹内侧核、乳头体复合体、上丘浅灰层、蓝斑、中缝背核中观察到高水平的结合。在额叶、额顶叶、颞叶、梨状皮质、齿状回、前嗅核、嗅结节、伏隔核壳、屏状核、外侧隔核、丘脑背外侧核、内侧缰核、下丘脑底核、丘脑 reunions核、下托、导水管周围灰质和脑桥中观察到中等程度的标记。在海马前部和内侧、嗅球、尾状壳核、伏隔核核心、内侧隔核、丘脑腹外侧核、丘脑腹后外侧核和丘脑背内侧核、外侧和内侧膝状体核、下丘脑区、黑质、腹侧被盖区和脚间核中观察到较低的结合位点密度。在脊髓背角和腹角中发现中等且相似的标记。胼胝体中未观察到标记。因此，ORL1受体似乎在大鼠脑的大脑皮质、边缘系统以及一些参与痛觉感知的区域中特别丰富。
