Yoshie H, Hirose Y, Suzuki T, Hara K
Department of Periodontology, Niigata University, School of Dentistry, Niigata, Japan.
Periodontal Clin Investig. 1996 Fall;18(2):31-8.
As a diagnostic test, "Periocheck" can detect the N-carbobenzoxyglycyl-glycy-arginyl peptidase that is produced by Treponema denticola, Porphyromonas gingivalis, and Bacteroides forsythus. The aim of this study was to clarify the relationship between peptidase activity and attachment loss. After Phase 1 and surgical therapy, a total of 111 sites from 47 adult periodontitis patients were divided into four groups according to peptidase activity (trypsin unit, TU): A, < 0.1 TU; B, 0.1-0.2 TU; C and D > or = 0.2 TU. All sites in groups A, B, and C were untreated, whereas both subgingival 3% hydrogen peroxide irrigation and 2% minocycline application were undertaken every 45 days throughout the experiment in group D. All subjects were recalled at 3-month intervals. Peptidase activity and clinical assessments were measured for the 18-month period. Significant attachment loss associated with high values of the peptidase activity was found through the experimental period in groups B and C. In contrast, no obvious change of attachment loss was found in groups A and D following low peptidase activity at 6, 12, and 18 months. The mean attachment loss throughout the 18-month period was 0.22 mm in group A, 1.04 mm in group B, 1.53 mm in group C, and -0.35 mm in group D. Probing depth and percentages of bleeding on probing significantly increased in group C, whereas they decreased in group D. This peptidase test displayed a 77.8% sensitivity and 68.6% specificity regarding the detection of > or = 1 mm attachment loss with a cut-off value of 0.1 TU. Multiple linear regression analysis showed a close relationship between peptidase activity and predictable attachment loss within a 12-month period. These findings suggest that this peptidase test is useful in identifying the risk sites for predictable attachment loss.
作为一种诊断测试，“牙周检查”可检测由齿垢密螺旋体、牙龈卟啉单胞菌和福赛坦氏拟杆菌产生的N-苄氧羰基甘氨酰-甘氨酰-精氨酰肽酶。本研究的目的是阐明肽酶活性与附着丧失之间的关系。在第一阶段和手术治疗后，将47例成人牙周炎患者的111个部位根据肽酶活性（胰蛋白酶单位，TU）分为四组：A组，<0.1 TU；B组，0.1 - 0.2 TU；C组和D组，≥0.2 TU。A、B和C组的所有部位均未接受治疗，而在整个实验过程中，D组每45天进行一次龈下3%过氧化氢冲洗和2%米诺环素应用。所有受试者每3个月复诊一次。在18个月的时间里测量肽酶活性和临床评估指标。在实验期间，发现B组和C组中肽酶活性高值与显著的附着丧失相关。相比之下，A组和D组在6、12和18个月时肽酶活性低，附着丧失无明显变化。在整个18个月期间，A组的平均附着丧失为0.22 mm，B组为1.04 mm，C组为1.53 mm，D组为 - 0.35 mm。C组的探诊深度和探诊出血百分比显著增加，而D组则下降。对于检测≥1 mm附着丧失，该肽酶测试以0.1 TU为临界值，显示出77.8%的敏感性和68.6%的特异性。多元线性回归分析表明，在12个月期间，肽酶活性与可预测的附着丧失之间存在密切关系。这些发现表明，这种肽酶测试有助于识别可预测附着丧失的风险部位。
