Prins J M, Schultsz C, Speelman P, van Deventer S J
Department of Internal Medicine, Academic Medical Center, Amsterdam, Netherlands.
FEMS Immunol Med Microbiol. 1996 Dec 31;16(3-4):283-9. doi: 10.1111/j.1574-695X.1996.tb00147.x.
We measured urinary endotoxin, IL-6 and IL-8 levels in 23 patients with gram-negative urosepsis. The endotoxin and cytokine levels showed a 100-1000 fold range. No correlation was found between levels of urinary endotoxin, and IL-6 or IL-8 levels. In all cases bacterial numbers were > or = 10(5) CFU ml-1 urine. The endotoxin content of the isolated microorganisms neither correlated with the urinary cytokine levels, nor with IL-6 and IL-8 levels obtained in vitro when 10(3) log-phase CFU of each of the bacteria were incubated with heparinized whole blood of three healthy donors. Neither the haemolysin phenotype of the bacteria, nor the presence of the P-pili gene was correlated with the cytokine response in vivo or in vitro. Other factors than known bacterial virulence factors apparently contribute to the wide variation in urinary cytokine levels in urinary tract infection.
我们检测了23例革兰阴性菌败血症患者的尿内毒素、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）水平。内毒素和细胞因子水平呈现出100至1000倍的范围。未发现尿内毒素水平与IL-6或IL-8水平之间存在相关性。所有病例中细菌数量均≥10⁵CFU/ml尿液。分离出的微生物的内毒素含量，既与尿细胞因子水平无关，也与在体外将每种细菌的10³对数期CFU与三名健康供体的肝素化全血孵育时获得的IL-6和IL-8水平无关。细菌的溶血素表型以及P菌毛基因的存在，均与体内或体外的细胞因子反应无关。除已知的细菌毒力因子外，其他因素显然导致了尿路感染患者尿细胞因子水平的广泛差异。
